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验证数据展示

  • WB analysis using 13893-1-AP

    WB analysis using 13893-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13893-1-AP (Synaptojanin 2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse small intestine using 13893-1-AP

    IHC staining of mouse small intestine using 13893-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 13893-1-AP (Synaptojanin 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 13893-1-AP

    IHC staining of human brain using 13893-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13893-1-AP (Synaptojanin 2 antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 13893-1-AP

    IHC staining of human brain using 13893-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 13893-1-AP (Synaptojanin 2 antibody at dilution of 1:50 (under 40x lens).

  • IF Staining of SH-SY5Y using 13893-1-AP

    IF Staining of SH-SY5Y using 13893-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Synaptojanin 2 antibody (13893-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Synaptojanin 2 Polyclonal antibody

Synaptojanin 2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13893-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

SYNJ2, Synaptojanin-2, Synaptic inositol 1,4,5-trisphosphate 5-phosphatase 2, KIAA0348, INPP5H

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IHC detected inhuman brain tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

13893-1-AP targets Synaptojanin 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 Synaptojanin 2 fusion protein Ag4855 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 synaptojanin 2
别名 SYNJ2, Synaptojanin-2, Synaptic inositol 1,4,5-trisphosphate 5-phosphatase 2, KIAA0348, INPP5H
计算分子量 1496 aa, 166 kDa
观测分子量143, 160 kDa
GenBank蛋白编号BC043277
基因名称 SYNJ2
Gene ID (NCBI) 8871
RRIDAB_2255784
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15056
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SYNJ2(Synaptojanin-2) is also named as KIAA0348 and belongs to the synaptojanin family. SYNJ2 encodes an inositol polyphosphate phosphatase that functions in recycling neurotransmitter vesicles and is implicated in spermatogenesis(PMID: 24103750). This protein has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for Synaptojanin 2 antibody 13893-1-APDownload protocol
IHC protocol for Synaptojanin 2 antibody 13893-1-APDownload protocol
IF protocol for Synaptojanin 2 antibody 13893-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Neuron

Neuronal mitochondria transport Pink1 mRNA via synaptojanin 2 to support local mitophagy.

Authors - Angelika B Harbauer
View Article
mouse

Front Cardiovasc Med

Macrophages-Related Genes Biomarkers in the Deterioration of Atherosclerosis.

Authors - Yue Zheng
View Article
mouseWB

Nat Metab

Insulin signalling regulates Pink1 mRNA localization via modulation of AMPK activity to support PINK1 function in neurons

Authors - J Tabitha Hees
View Article