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SYNJ2 Polyclonal antibody

SYNJ2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30391-1-AP

Print datasheet

Synonyms

synaptojanin 2, Synaptojanin-2, Synaptic inositol 1,4,5-trisphosphate 5-phosphatase 2, KIAA0348, INPP5H

验证数据展示

  • WB analysis using 30391-1-AP

    WB analysis using 30391-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30391-1-AP (SYNJ2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 30391-1-AP

    WB analysis using 30391-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30391-1-AP (SYNJ2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse small intestine using 30391-1-AP

    IHC staining of mouse small intestine using 30391-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30391-1-AP (SYNJ2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse small intestine using 30391-1-AP

    IHC staining of mouse small intestine using 30391-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30391-1-AP (SYNJ2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U-251 using 30391-1-AP

    IF Staining of U-251 using 30391-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using SYNJ2 antibody (30391-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IHC detected inmouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30391-1-AP targets SYNJ2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SYNJ2 fusion protein Ag33002 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name synaptojanin 2
Synonyms synaptojanin 2, Synaptojanin-2, Synaptic inositol 1,4,5-trisphosphate 5-phosphatase 2, KIAA0348, INPP5H
Calculated Molecular Weight 1496 aa, 166 kDa
Observed Molecular Weight143 kDa, 160 kDa
GenBank Accession NumberBC043277
Gene Symbol SYNJ2
Gene ID (NCBI) 8871
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15056
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SYNJ2(Synaptojanin-2) is also named as KIAA0348 and belongs to the synaptojanin family. SYNJ2 encodes an inositol polyphosphate phosphatase that functions in recycling neurotransmitter vesicles and is implicated in spermatogenesis(PMID: 24103750). This protein has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for SYNJ2 antibody 30391-1-APDownload protocol
IHC protocol for SYNJ2 antibody 30391-1-APDownload protocol
IF protocol for SYNJ2 antibody 30391-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols