SYNPO Polyclonal antibody

SYNPO Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 21064-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA and More (1)

Synaptopodin, snaptopodin, KIAA1029

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue, mouse kidney tissue
Planning an IHC experiment? We recommend our IHCeasy SYNPO Ready-To-Use IHC Kit. SYNPO primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21064-1-AP targets SYNPO in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 SYNPO fusion protein Ag15089 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 synaptopodin
别名 Synaptopodin, snaptopodin, KIAA1029
计算分子量 929 aa, 99 kDa
观测分子量 99-115 kDa
GenBank蛋白编号BC146665
基因名称 SYNPO
Gene ID (NCBI) 11346
RRIDAB_10733120
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N3V7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)

实验方案

Product Specific Protocols
WB protocol for SYNPO antibody 21064-1-APDownload protocol
IHC protocol for SYNPO antibody 21064-1-APDownload protocol
IF protocol for SYNPO antibody 21064-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

J Extracell Vesicles

Quantification of urinary podocyte-derived migrasomes for the diagnosis of kidney disease

Authors - Rong Yang
humanIF

Biofabrication

A glomerulus chip with spherically twisted cell-laden hollow fibers as glomerular capillary tufts

Authors - Zhilin Dai
humanIF

Theranostics

Renal tubular Bim mediates the tubule-podocyte crosstalk via NFAT2 to induce podocyte cytoskeletal dysfunction.

Authors - Chunmei Xu
human,mouseIF

Diabetes

Deficiency of Mitochondrial Glycerol 3-Phosphate Dehydrogenase Exacerbates Podocyte Injury and the Progression of Diabetic Kidney Disease.

Authors - Hua Qu
mouseIF

Free Radic Biol Med

P2X7R/AKT/mTOR signaling mediates high glucose-induced decrease in podocyte autophagy

Authors - Cheng Qian
mouseWB,IF

Mol Ther Nucleic Acids

Inhibition of the lncRNA MIAT prevents podocyte injury and mitotic catastrophe in diabetic nephropathy.

Authors - Ziyang Wang
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