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SYNPO Recombinant antibody, PBS Only

SYNPO Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4K5

Cat no : 80721-1-PBS

Print datasheet

Synonyms

Synaptopodin, snaptopodin, KIAA1029, 4K5

验证数据展示

  • IHC staining of human kidney using 80721-1-RR (same clone as 80721-1-PBS)

    IHC staining of human kidney using 80721-1-RR (same clone as 80721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SYNPO antibody included
  • WB analysis using 80721-1-RR (same clone as 80721-1-PBS)

    WB analysis using 80721-1-RR (same clone as 80721-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80721-1-RR (SYNPO antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 80721-1-RR (same clone as 80721-1-PBS)

    IHC staining of human kidney using 80721-1-RR (same clone as 80721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SYNPO antibody included
  • IHC staining of rat kidney using 80721-1-RR (same clone as 80721-1-PBS)

    IHC staining of rat kidney using 80721-1-RR (same clone as 80721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SYNPO antibody included
  • IHC staining of rat kidney using 80721-1-RR (same clone as 80721-1-PBS)

    IHC staining of rat kidney using 80721-1-RR (same clone as 80721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SYNPO antibody included
  • IHC staining of mouse kidney using 80721-1-RR (same clone as 80721-1-PBS)

    IHC staining of mouse kidney using 80721-1-RR (same clone as 80721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SYNPO antibody included
  • IHC staining of mouse kidney using 80721-1-RR (same clone as 80721-1-PBS)

    IHC staining of mouse kidney using 80721-1-RR (same clone as 80721-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 80721-1-RR (SYNPO antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80721-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SYNPO antibody included
  • Affinity and Kinetic Characterization of 80721-1-RR

    Affinity and Kinetic Characterization of 80721-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80721-1-RR against Human SYNPO were performed. The affinity constant is 1.38 nM.

Planning an IHC experiment? We recommend our IHCeasy SYNPO Ready-To-Use IHC Kit. SYNPO primary antibody included.

产品信息

80721-1-PBS targets SYNPO in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen SYNPO fusion protein Ag31287 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name synaptopodin
Synonyms Synaptopodin, snaptopodin, KIAA1029, 4K5
Calculated Molecular Weight 929 aa, 99 kDa
Observed Molecular Weight100 kDa
GenBank Accession NumberBC146665
Gene Symbol SYNPO
Gene ID (NCBI) 11346
RRIDAB_2918907
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8N3V7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Synaptopodin is a actin-associated protein expressed in mature dendritic spines and renal podocytes. Several isoforms of synaptopodin have been reported: the 100 kDa neural form and 110 kDa renal form. 44-45 kDa may represent the degradation product of synaptopodin. As a podocyte specific protein, synaptopodin expression level was decreased in kidney but increased in the urinary podocytes from patients with preeclampsia, indicating that synaptopodin is a useful marker for preeclampsia. (15841212, 10555072)