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验证数据展示

  • WB analysis using 21480-1-AP

    WB analysis using 21480-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21480-1-AP (SYNPO2L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 21480-1-AP

    IHC staining of mouse heart using 21480-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21480-1-AP (SYNPO2L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 21480-1-AP

    IF Staining of HepG2 using 21480-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SYNPO2L antibody (21480-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse heart using 21480-1-AP

    IHC staining of mouse heart using 21480-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21480-1-AP (SYNPO2L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SYNPO2L Polyclonal antibody

SYNPO2L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21480-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

Synaptopodin 2-like protein, Synaptopodin 2 like protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, PC-3 cells, rat testis tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

21480-1-AP targets SYNPO2L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 SYNPO2L fusion protein Ag15683 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 synaptopodin 2-like
别名 Synaptopodin 2-like protein, Synaptopodin 2 like protein
计算分子量 977 aa, 102 kDa
观测分子量 79 kDa
GenBank蛋白编号BC142635
基因名称 SYNPO2L
Gene ID (NCBI) 79933
RRIDAB_10860260
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H987
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SYNPO2L belongs to the synaptopodin family and is an actin-associated protein that may play a role in modulating actin-based shape. SYNPO2L is involved in the positive regulation of Rho protein signal transduction and the positive regulation of stress fiber assembly. Loss-of-function variants in the SYNPO2L gene are associated with atrial fibrillation (PMID: 33768119).

实验方案

Product Specific Protocols
WB protocol for SYNPO2L antibody 21480-1-APDownload protocol
IHC protocol for SYNPO2L antibody 21480-1-APDownload protocol
IF protocol for SYNPO2L antibody 21480-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Cells

Direct Binding of Synaptopodin 2-Like Protein to Alpha-Actinin Contributes to Actin Bundle Formation in Cardiomyocytes

Authors - Hiroshi Yamada
View Article