Synaptophysin Monoclonal antibody
Synaptophysin Monoclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Mouse / IgG3
Reactivity
human, mouse, rat, pig, rabbit
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
5B3F2
验证数据展示
经过测试的应用
Positive WB detected in | fetal human brain tissue, human brain tissue, pig brain tissue, SH-SY5Y cells |
Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | PC-12 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 60191-1-Ig is Synaptophysin Fusion Protein expressed in E. coli.
Tested Applications | WB, IF, IHC, ELISA Application Description |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human, mouse, rat, pig, rabbit |
Cited Reactivity | human, mouse, rat |
Immunogen | Synaptophysin fusion protein Ag11803 种属同源性预测 |
Host / Isotype | Mouse / IgG3 |
Class | Monoclonal |
Type | Antibody |
Full Name | synaptophysin |
Synonyms | synaptophysin, SYP |
Calculated Molecular Weight | 313 aa, 34 kDa |
Observed Molecular Weight | 38 kDa |
GenBank Accession Number | BC064550 |
Gene Symbol | Synaptophysin |
Gene ID (NCBI) | 6855 |
RRID | AB_10915965 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P08247 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Synaptophysin antibody 60191-1-Ig | Download protocol |
IHC protocol for Synaptophysin antibody 60191-1-Ig | Download protocol |
IF protocol for Synaptophysin antibody 60191-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Brain Behav Immun l-Cysteine suppresses hypoxia-ischemia injury in neonatal mice by reducing glial activation, promoting autophagic flux and mediating synaptic modification via H2S formation. | ||
Nat Protoc Nanoscale imaging of clinical specimens using conventional and rapid-expansion pathology. | ||
Aging Cell Gamma frequency light flicker regulates amyloid precursor protein trafficking for reducing β-amyloid load in Alzheimer's disease model. | ||
Cell Death Dis Phosphorylation of Parkin at serine 131 by p38 MAPK promotes mitochondrial dysfunction and neuronal death in mutant A53T α-synuclein model of Parkinson's disease. | ||
FASEB J Silencing of circIgf1r plays a protective role in neuronal injury via regulating astrocyte polarization during epilepsy. | ||
Chemosphere Arsenic exposure via drinking water during pregnancy and lactation induces autism-like behaviors in male offspring mice. |