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Synaptotagmin-13 Polyclonal antibody

Synaptotagmin-13 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat No : 18115-1-AP

Print datasheet

Synonyms

SytXIII, SYT13, Synaptotagmin XIII, KIAA1427

验证数据展示

  • WB analysis of HepG2 using 18115-1-AP

    WB analysis of HepG2 using 18115-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18115-1-AP (Synaptotagmin-13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 18115-1-AP

    IHC staining of human stomach cancer using 18115-1-AP

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 18115-1-AP (Synaptotagmin-13 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHepG2 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

18115-1-AP targets Synaptotagmin-13 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen Synaptotagmin-13 fusion protein Ag12599 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name synaptotagmin XIII
Synonyms SytXIII, SYT13, Synaptotagmin XIII, KIAA1427
Calculated Molecular Weight 426 aa, 47 kDa
Observed Molecular Weight 47 kDa, 66 kDa
GenBank Accession NumberBC093830
Gene Symbol Synaptotagmin-13
Gene ID (NCBI) 57586
RRIDAB_2199453
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7L8C5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Synaptotagmin-13 is a member of the synaptotagmin family of proteins which are known as membrane trafficking proteins. Synaptotagmin-13 is composed of the canonical domains of synaptotagmins that include an N-terminal transmembrane region, two C-terminal cytoplasmic C2-domains, and a connecting sequence between the transmembrane region and the C2-domains (PMID: 11211934). Synaptotagmin-13 is highly expressed in brain and may be involved in transport vesicle docking to the plasma membrane.

实验方案

Product Specific Protocols
WB protocol for Synaptotagmin-13 antibody 18115-1-APDownload protocol
IHC protocol for Synaptotagmin-13 antibody 18115-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Brain

Key role of SMN/SYNCRIP and RNA-Motif 7 in spinal muscular atrophy: RNA-Seq and motif analysis of human motor neurons.

Authors - Federica Rizzo
View Article