验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, mouse cerebellum tissue, rat brain tissue, Neuro-2a cells |
Positive IF-P detected in | mouse cerebellum tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
21245-1-AP targets SYT2 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IF-P, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | SYT2 fusion protein Ag15744 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | synaptotagmin II |
别名 | SytII, Synaptotagmin-2, Synaptotagmin II |
计算分子量 | 419 aa, 47 kDa |
观测分子量 | 70 kDa |
GenBank蛋白编号 | BC100815 |
基因名称 | SYT2 |
Gene ID (NCBI) | 127833 |
RRID | AB_3085646 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q8N9I0 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SYT2 (Synaptotagmin-2) a synaptic vesicle membrane protein, functions as a calcium sensor in vesicular trafficking and exocytosis. SYT2 is also plays a role in dendrite formation by melanocytes (PMID: 23999003). Mutations in SYT2 are associated with myasthenic syndrome, presynaptic, congenital, with or without motor neuropathy. SYT2 mutations cause a complex presynaptic congenital myasthenic syndrome (PMID: 26519543).
实验方案
Product Specific Protocols | |
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WB protocol for SYT2 antibody 21245-1-AP | Download protocol |
IF protocol for SYT2 antibody 21245-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |