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验证数据展示

  • WB analysis using 21245-1-AP

    WB analysis using 21245-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21245-1-AP (SYT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse cerebellum using 21245-1-AP

    IF Staining of mouse cerebellum using 21245-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using SYT2 antibody (21245-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse cerebellum using 21245-1-AP

    IF Staining of mouse cerebellum using 21245-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using SYT2 antibody (21245-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

SYT2 Polyclonal antibody

SYT2 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 21245-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Synonyms

SytII, Synaptotagmin-2, Synaptotagmin II

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue, Neuro-2a cells
Positive IF-P detected inmouse cerebellum tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21245-1-AP targets SYT2 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SYT2 fusion protein Ag15744 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name synaptotagmin II
Synonyms SytII, Synaptotagmin-2, Synaptotagmin II
Calculated Molecular Weight 419 aa, 47 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC100815
Gene Symbol SYT2
Gene ID (NCBI) 127833
RRIDAB_3085646
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N9I0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SYT2 (Synaptotagmin-2) a synaptic vesicle membrane protein, functions as a calcium sensor in vesicular trafficking and exocytosis. SYT2 is also plays a role in dendrite formation by melanocytes (PMID: 23999003). Mutations in SYT2 are associated with myasthenic syndrome, presynaptic, congenital, with or without motor neuropathy. SYT2 mutations cause a complex presynaptic congenital myasthenic syndrome (PMID: 26519543).

实验方案

Product Specific Protocols
WB protocol for SYT2 antibody 21245-1-APDownload protocol
IF protocol for SYT2 antibody 21245-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols