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Synaptotagmin-8 Polyclonal antibody

Synaptotagmin-8 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20671-1-AP

Print datasheet

Synonyms

SytVIII, SYT8, Synaptotagmin VIII

验证数据展示

  • WB analysis using 20671-1-AP

    WB analysis using 20671-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20671-1-AP (Synaptotagmin-8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of Caco-2 using 20671-1-AP

    IF Staining of Caco-2 using 20671-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using Synaptotagmin-8 antibody (20671-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman placenta tissue, mouse placenta tissue
Positive IF/ICC detected inCaco-2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20671-1-AP targets Synaptotagmin-8 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name synaptotagmin VIII
Synonyms SytVIII, SYT8, Synaptotagmin VIII
Calculated Molecular Weight 44 kDa
Observed Molecular Weight40 kDa, 50 kDa
GenBank Accession NumberNM_138567
Gene Symbol SYT8
Gene ID (NCBI) 90019
RRIDAB_3085610
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NBV8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). SYT8 (synaptotagmin-8) is related to insulin secretion, and its expression is positively regulated by the insulin promoter (PMID: 21336277). Due to alternative splicing, SYT8 may produce proteins with molecular weights between 25 and 40 to 44 kDa. An additional band of 50 kDa could also be detected (PMID: 16386321)

实验方案

Product Specific Protocols
WB protocol for Synaptotagmin-8 antibody 20671-1-APDownload protocol
IF protocol for Synaptotagmin-8 antibody 20671-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols