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验证数据展示

  • WB analysis using 20671-1-AP

    WB analysis using 20671-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20671-1-AP (Synaptotagmin-8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of Caco-2 using 20671-1-AP

    IF Staining of Caco-2 using 20671-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using Synaptotagmin-8 antibody (20671-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Synaptotagmin-8 Polyclonal antibody

Synaptotagmin-8 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 20671-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

SytVIII, SYT8, Synaptotagmin VIII

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue, mouse placenta tissue
Positive IF/ICC detected inCaco-2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20671-1-AP targets Synaptotagmin-8 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 synaptotagmin VIII
别名 SytVIII, SYT8, Synaptotagmin VIII
计算分子量 44 kDa
观测分子量40 kDa, 50 kDa
GenBank蛋白编号NM_138567
基因名称 SYT8
Gene ID (NCBI) 90019
RRIDAB_3085610
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8NBV8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). SYT8 (synaptotagmin-8) is related to insulin secretion, and its expression is positively regulated by the insulin promoter (PMID: 21336277). Due to alternative splicing, SYT8 may produce proteins with molecular weights between 25 and 40 to 44 kDa. An additional band of 50 kDa could also be detected (PMID: 16386321)

实验方案

Product Specific Protocols
WB protocol for Synaptotagmin-8 antibody 20671-1-APDownload protocol
IF protocol for Synaptotagmin-8 antibody 20671-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols