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Synaptotagmin-9 Monoclonal antibody

Synaptotagmin-9 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3D2F2

Cat no : 68430-1-Ig

Print datasheet

Synonyms

SytIX, SYT9, Synaptotagmin IX, 3D2F2

验证数据展示

  • WB analysis using 68430-1-Ig

    WB analysis using 68430-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68430-1-Ig (Synaptotagmin-9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

经过测试的应用

Positive WB detected inrabbit retina tissue, rat retina tissue, mouse retina tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68430-1-Ig targets Synaptotagmin-9 in WB, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat, rabbit
Immunogen Synaptotagmin-9 fusion protein Ag33194 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name synaptotagmin IX
Synonyms SytIX, SYT9, Synaptotagmin IX, 3D2F2
Calculated Molecular Weight 491 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC029605
Gene Symbol Synaptotagmin-9
Gene ID (NCBI) 143425
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ86SS6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). Synaptotagmin-9 (SYT9) is a tandem C2 domain Ca 2+ sensor for exocytosis in neuroendocrine cells (PMID: 36732068).

实验方案

Product Specific Protocols
WB protocol for Synaptotagmin-9 antibody 68430-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols