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RISC Monoclonal antibody

RISC Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C6A7

Cat no : 60086-1-Ig

Print datasheet

Synonyms

2410018F01Rik, 4833411K15Rik, AI957256, Risc, Scpep1, serine carboxypeptidase 1

验证数据展示

  • WB analysis of human testis using 60086-1-Ig

    WB analysis of human testis using 60086-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 60086-1-Ig (RISC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 60086-1-Ig

    IHC staining of human pancreas cancer using 60086-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60086-1-Ig (RISC antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 60086-1-Ig

    IHC staining of human pancreas cancer using 60086-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60086-1-Ig (RISC antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of C6 using 60086-1-Ig

    IF Staining of C6 using 60086-1-Ig

    Immunofluorescent analysis of C6 cells using 60086-1-Ig (RISC antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

经过测试的应用

Positive WB detected inhuman testis tissue, human heart tissue, MCF-7 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inC6 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

60086-1-Ig targets RISC in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen RISC fusion protein Ag0614 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name serine carboxypeptidase 1
Synonyms 2410018F01Rik, 4833411K15Rik, AI957256, Risc, Scpep1, serine carboxypeptidase 1
Calculated Molecular Weight 452 aa, 51 kDa
Observed Molecular Weight 35-40 kDa/50-60 kDa
GenBank Accession NumberAF330052
Gene Symbol Scpep1
Gene ID (NCBI) 74617
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
UNIPROT IDQ920A5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Scpep1(Retinoid-inducible serine carboxypeptidase) is a 51 kDa protein which is also named as RISC and belongs to the peptidase S10 family. This protein may be involved in vascular wall and kidney homeostasis.

实验方案

Product Specific Protocols
WB protocol for RISC antibody 60086-1-IgDownload protocol
IHC protocol for RISC antibody 60086-1-IgDownload protocol
IF protocol for RISC antibody 60086-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Autophagy

Donor MSCs release apoptotic bodies to improve myocardial infarction via autophagy regulation in recipient cells.

Authors - Huan Liu
View Article