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Serum amyloid P component Polyclonal antibody

Serum amyloid P component Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 31813-1-AP

Print datasheet

Synonyms

APCS, SAP, PTX2, amyloid P component, serum, 9.5S alpha-1-glycoprotein

验证数据展示

  • WB analysis using 31813-1-AP

    WB analysis using 31813-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31813-1-AP (Serum amyloid P component antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 31813-1-AP

    IHC staining of human stomach cancer using 31813-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31813-1-AP (Serum amyloid P component antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse liver using 31813-1-AP

    IF Staining of mouse liver using 31813-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Serum amyloid P component antibody (31813-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse liver using 31813-1-AP

    IF Staining of mouse liver using 31813-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Serum amyloid P component antibody (31813-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman plasma
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31813-1-AP targets Serum amyloid P component in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name amyloid P component, serum
Synonyms APCS, SAP, PTX2, amyloid P component, serum, 9.5S alpha-1-glycoprotein
Calculated Molecular Weight 223 aa, 25 kDa
Observed Molecular Weight 27-29 kDa
GenBank Accession NumberBC007058
Gene Symbol Serum amyloid P component
Gene ID (NCBI) 325
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP02743
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Serum amyloid P component (SAP), a member of the pentraxin family, is a constitutive serum protein that is synthesized by hepatocytes. It is an acute phase protein, structurally related to C-reactive protein. SAP is a calcium-dependent ligand-binding protein, which can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

实验方案

Product Specific Protocols
WB protocol for Serum amyloid P component antibody 31813-1-APDownload protocol
IHC protocol for Serum amyloid P component antibody 31813-1-APDownload protocol
IF protocol for Serum amyloid P component antibody 31813-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols