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验证数据展示

  • WB analysis of A549 using 28594-1-AP

    WB analysis of A549 using 28594-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28594-1-AP (Siglec-15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Siglec-15 Polyclonal antibody

Siglec-15 Polyclonal Antibody for WB, ELISA
Cat No. 28594-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

CD33 antigen like 3, CD33L3, HsT1361, SIGLEC 15, SIGLEC15

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, NCI-H1299 cells, PC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28594-1-AP targets Siglec-15 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 Siglec-15 fusion protein Ag29829 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sialic acid binding Ig-like lectin 15
别名 CD33 antigen like 3, CD33L3, HsT1361, SIGLEC 15, SIGLEC15
计算分子量 36 kDa
观测分子量38-40 kDa
GenBank蛋白编号NM_213602
基因名称 Siglec-15
Gene ID (NCBI) 284266
RRIDAB_2918178
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6ZMC9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sialic acid binding Ig-like lectin 15 (Siglec-15) is a member of the Siglec family of glycan-recognition proteins. Siglec-15 is a type-I transmembrane protein consisting of two immunoglobulin (Ig)-like domains, a transmembrane domain, and a short cytoplasmic tail (PMID: 17483134). It is mainly expressed on a subset of myeloid lineage cells and can be upregulated in many cancers (PMID: 32939323). Siglec-15 has been reported as a immune suppressor and a potential target for normalization cancer immunotherapy (PMID: 30833750). Siglec-15 has a calculated molecular weight of 36 kDa. It has been detected at 38-40 kDa due to glycosylation (PMID: 32921411).

实验方案

Product Specific Protocols
WB protocol for Siglec-15 antibody 28594-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols