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Siglec-9 Recombinant antibody

Siglec-9 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241799E8

Cat no : 84683-4-RR

Print datasheet

Synonyms

SIGLEC9, Siglec 9, Sialic acid-binding Ig-like lectin 9, Protein FOAP 9, CD329

验证数据展示

  • WB analysis using 84683-4-RR

    WB analysis using 84683-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84683-4-RR (Siglec-9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84683-4-RR targets Siglec-9 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name sialic acid binding Ig-like lectin 9
Synonyms SIGLEC9, Siglec 9, Sialic acid-binding Ig-like lectin 9, Protein FOAP 9, CD329
Calculated Molecular Weight50 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberNM_014441.3
Gene Symbol Siglec-9
Gene ID (NCBI) 27180
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9Y336-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sialic acid binding Ig-like lectin 9 (Siglec-9), also known as CD329, is a member of the Siglec family of glycan-recognition proteins. Siglec-9 is a type-I transmembrane protein consisting of an N-terminal extracellular region that contains an N-terminal V-set domain and two C2-set domains, a transmembrane region, and an intracellular domain with an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an ITIM-like motif (PMID: 10801862). It is expressed quite broadly among human blood leukocytes, including monocytes, neutrophils, B cells, NK cells, and minor subsets of T cells (PMID: 32322597). Siglec-9 functions as an inhibitory immune checkpoint and can be targeted to enhance therapeutic antitumor immunity (PMID: 34155121; 37460871).

实验方案

Product Specific Protocols
WB protocol for Siglec-9 antibody 84683-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols