验证数据展示
经过测试的应用
Positive WB detected in | ROS1728 cells, SK-N-SH cells, SH-SY5Y cells, pig brain tissue, rabbit brain, rat brain tissue, mouse brain tissue |
Positive IHC detected in | mouse brain tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue |
Positive IF/ICC detected in | SH-SY5Y cells, HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68007-1-Ig targets Sortilin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
经测试应用 | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
经测试反应性 | human, mouse, rat, pig |
免疫原 | Sortilin fusion protein Ag28043 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | sortilin 1 |
别名 | SORT1, NTR3, NT3, Neurotensin receptor 3, Gp95 |
计算分子量 | 831 aa, 92 kDa |
观测分子量 | 95-100 kDa |
GenBank蛋白编号 | BC023542 |
基因名称 | Sortilin |
Gene ID (NCBI) | 6272 |
ENSEMBL Gene ID | ENSG00000134243 |
RRID | AB_2918754 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q99523 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sortilin 1 (SORT1), is a trans-Golgi network (TGN) transmembrane protein and is a member of the Vps10p domain receptor family. SORT1 binds a number of unrelated ligands that participate in a wide range of cellular processes. It functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface.
实验方案
Product Specific Protocols | |
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WB protocol for Sortilin antibody 68007-1-Ig | Download protocol |
IHC protocol for Sortilin antibody 68007-1-Ig | Download protocol |
IF protocol for Sortilin antibody 68007-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |