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Sortilin Monoclonal antibody

Sortilin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2B11B9

Cat no : 68007-1-Ig

Print datasheet

Synonyms

SORT1, NTR3, NT3, Neurotensin receptor 3, Gp95

验证数据展示

  • WB analysis using 68007-1-Ig

    WB analysis using 68007-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68007-1-Ig (Sortilin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 68007-1-Ig

    IHC staining of mouse brain using 68007-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68007-1-Ig (Sortilin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SORT1 antibody included
  • IHC staining of mouse brain using 68007-1-Ig

    IHC staining of mouse brain using 68007-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68007-1-Ig (Sortilin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SORT1 antibody included
  • IF Staining of mouse brain using 68007-1-Ig

    IF Staining of mouse brain using 68007-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Sortilin antibody (68007-1-Ig, Clone: 2B11B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of SH-SY5Y using 68007-1-Ig

    IF Staining of SH-SY5Y using 68007-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Sortilin antibody (68007-1-Ig, Clone: 2B11B9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

  • IF Staining of SH-SY5Y using 68007-1-Ig

    IF Staining of SH-SY5Y using 68007-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Sortilin antibody (68007-1-Ig, Clone: 2B11B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inROS1728 cells, SK-N-SH cells, SH-SY5Y cells, pig brain tissue, rabbit brain, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy SORT1 Ready-To-Use IHC Kit. SORT1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68007-1-Ig targets Sortilin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen Sortilin fusion protein Ag28043 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name sortilin 1
Synonyms SORT1, NTR3, NT3, Neurotensin receptor 3, Gp95
Calculated Molecular Weight 831 aa, 92 kDa
Observed Molecular Weight95-100 kDa
GenBank Accession NumberBC023542
Gene Symbol Sortilin
Gene ID (NCBI) 6272
ENSEMBL Gene IDENSG00000134243
RRIDAB_2918754
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99523
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sortilin 1 (SORT1), is a trans-Golgi network (TGN) transmembrane protein and is a member of the Vps10p domain receptor family. SORT1 binds a number of unrelated ligands that participate in a wide range of cellular processes. It functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface.

实验方案

Product Specific Protocols
WB protocol for Sortilin antibody 68007-1-IgDownload protocol
IHC protocol for Sortilin antibody 68007-1-IgDownload protocol
IF protocol for Sortilin antibody 68007-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols