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Stomatin Monoclonal antibody

Stomatin Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A4D6

Cat No : 67894-1-Ig

Print datasheet

Synonyms

BND7, EPB7, EPB72, Protein 7.2b, STOM, stomatin

验证数据展示

  • WB analysis of human placenta using 67894-1-Ig

    WB analysis of human placenta using 67894-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67894-1-Ig (Stomatin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 67894-1-Ig

    IHC staining of human lung cancer using 67894-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67894-1-Ig (Stomatin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman placenta tissue, HeLa cells, human peripheral blood platelets, human testis tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67894-1-Ig targets Stomatin in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA Application Description
Tested Reactivity Human
Immunogen Stomatin fusion protein Ag2676 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name stomatin
Synonyms BND7, EPB7, EPB72, Protein 7.2b, STOM, stomatin
Calculated Molecular Weight 288 aa, 32 kDa
Observed Molecular Weight32 kDa
GenBank Accession NumberBC010703
Gene Symbol STOM
Gene ID (NCBI) 2040
RRIDAB_2918650
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP27105
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

STOM, also named as BND7, EPB72, Stomatin and Protein 7.2b, belongs to the band 7/mec-2 family. It is thought to regulate cation conductance. STOM may regulate ACCN1 and ACCN3 gating. STOM is a membrane protein involved in regulation of monovalent cation transport through lipid membranes. Interestingly, STOM (which has a structure similar to caveolin) is a major lipid-raft component of erythrocytes. It may have a role in membrane microdomains modulation leading to membrane budding and MPs release.(PMID:19138258)

实验方案

Product Specific Protocols
WB protocol for Stomatin antibody 67894-1-IgDownload protocol
IHC protocol for Stomatin antibody 67894-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols