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验证数据展示

  • WB analysis of human placenta using 67894-1-Ig

    WB analysis of human placenta using 67894-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67894-1-Ig (Stomatin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 67894-1-Ig

    IHC staining of human lung cancer using 67894-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67894-1-Ig (Stomatin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STOM antibody included

Stomatin Monoclonal antibody

Stomatin Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67894-1-Ig

产品说明书

CloneNo. 1A4D6

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IHC, ELISA

别名

BND7, EPB7, EPB72, Protein 7.2b, STOM, stomatin

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue, HeLa cells, human peripheral blood platelets, human testis tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy STOM Ready-To-Use IHC Kit. STOM primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67894-1-Ig targets Stomatin in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 Stomatin fusion protein Ag2676 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 stomatin
别名 BND7, EPB7, EPB72, Protein 7.2b, STOM, stomatin
计算分子量 288 aa, 32 kDa
观测分子量32 kDa
GenBank蛋白编号BC010703
基因名称 Stomatin
Gene ID (NCBI) 2040
RRIDAB_2918650
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP27105
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

STOM, also named as BND7, EPB72, Stomatin and Protein 7.2b, belongs to the band 7/mec-2 family. It is thought to regulate cation conductance. STOM may regulate ACCN1 and ACCN3 gating. STOM is a membrane protein involved in regulation of monovalent cation transport through lipid membranes. Interestingly, STOM (which has a structure similar to caveolin) is a major lipid-raft component of erythrocytes. It may have a role in membrane microdomains modulation leading to membrane budding and MPs release.(PMID:19138258)

实验方案

Product Specific Protocols
WB protocol for Stomatin antibody 67894-1-IgDownload protocol
IHC protocol for Stomatin antibody 67894-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols