ChromoTek Strep-NanoTrap Agarose
The ChromoTek Strep-NanoTrap Agarose consists of an anti-Strep-tag® Nanobody/VHH, which is coupled to agarose beads. It can be used for the immunoprecipitation of proteins fused to the peptide SAWSHPQFEK, also known as Strep-Tag® from cell extracts of various organisms.
Specificity
Strep-Tag® (sequence: SAWSHPQFEK)
Applications
IP, Co-IP
Type
Nanobody
Conjugate
Agarose
Cat No : qta
Synonyms
Validation Data Gallery
Product Information
The ChromoTek Strep-NanoTrap Agarose consists of an anti-Strep-tag® Nanobody/VHH, which is coupled to agarose beads. It can be used for the immunoprecipitation of proteins fused to the peptide SAWSHPQFEK, also known as Strep-Tag® from cell extracts of various organisms.
Description | Immunoprecipitation of Strep-tagged proteins with anti-Strep-tag Nanobody conjugated to agarose beads. • Fast, reliable & efficient one-step immunoprecipitation • Ready-to-use • No heavy & light antibody chains • Stable under harsh washing conditions • Suitable for downstream mass spec analysis |
Applications | IP, Co-IP |
Specificity/Target | Binds specifically to the peptide sequence SAWSHPQFEK, also known as Strep-Tag® or Strep-TagII®, fused to a protein of interest at N- or C-terminal position. In addition, this trap binds to the peptide sequence SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK, also known as Twin-Strep-Tag®, fused to a protein of interest at N- or C-terminal position. |
Binding Capacity | 25 ug of recombinant SAWSHPQFEK-tagged protein (~30kDa) per 25 uL bead slurry |
Conjugate | Agarose beads; ~90 um (cross-linked 4% agarose beads) |
Elution buffer | 2x SDS-sample buffer (Lämmli), 200 mM glycine pH 2.5 or with SAWSHPQFEK peptide |
Wash Buffer Compatibility | 1M NaCl, 5 mM DTT, 5 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0.1% SDS, 2-3 M Urea |
Type | Nanobody |
Class | Recombinant |
Host | Alpaca |
Affinity (KD) | 480 nM for N-terminal Strep-Tag® and 600 nM for C-terminal Strep-Tag® |
Compatibility with mass spectrometry | The Strep-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
RRID | AB_3107051 |
Storage Buffer | 20% ethanol |
Storage Condition | Shipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. Do not freeze! |