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Supervillin Polyclonal antibody

Supervillin Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27524-1-AP

Print datasheet

Synonyms

SVIL

验证数据展示

  • WB analysis using 27524-1-AP

    WB analysis using 27524-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27524-1-AP (Supervillin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA431 cells, HepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27524-1-AP targets Supervillin in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Supervillin fusion protein Ag26196 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name supervillin
Synonyms SVIL
Calculated Molecular Weight 248 kDa
Observed Molecular Weight 205 kDa
GenBank Accession NumberNM_003174
Gene Symbol SVIL
Gene ID (NCBI) 6840
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95425
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Supervillin (SVIL) belongs to the villin/gelsolin superfamily of actin-binding proteins involved in many cellular processes. A non-muscle-specific form of Supervillin activates androgen receptor activity. Supervillin another isoform, called Archvillin, is expressed in skeletal and cardiac muscle tissue where it plays a role in both muscle fiber structure and signaling. Mutations in Supervillin are associated with myofibrillar Myopathy10 (MFM10), which causes myopathy with myofibrillar disorganization and autophagic vacuoles(PMID: 9867483, 32779703).

实验方案

Product Specific Protocols
WB protocol for Supervillin antibody 27524-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols