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SYNGR1 Polyclonal antibody

SYNGR1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29734-1-AP

Print datasheet

Synonyms

Synaptogyrin-1, synaptogyrin 1

验证数据展示

  • WB analysis using 29734-1-AP

    WB analysis using 29734-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29734-1-AP (SYNGR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 29734-1-AP

    WB analysis using 29734-1-AP

    Unboiled rat brain tissue was subjected to SDS PAGE followed by western blot with 29734-1-AP (SYNGR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 29734-1-AP

    IHC staining of mouse brain using 29734-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29734-1-AP (SYNGR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 29734-1-AP

    IHC staining of mouse brain using 29734-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29734-1-AP (SYNGR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 29734-1-AP

    IHC staining of rat brain using 29734-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 29734-1-AP (SYNGR1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 29734-1-AP

    IHC staining of rat brain using 29734-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 29734-1-AP (SYNGR1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inunboiled rat brain tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29734-1-AP targets SYNGR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SYNGR1 fusion protein Ag31214 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name synaptogyrin 1
Synonyms Synaptogyrin-1, synaptogyrin 1
Calculated Molecular Weight 25 kDa
Observed Molecular Weight28-30 kDa
GenBank Accession NumberBC000731
Gene Symbol SYNGR1
Gene ID (NCBI) 9145
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43759
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Synaptogyrins are one of the most abundant vesicle components and are involved in the regulation of neurotransmitter release and synaptic plasticity. Synaptogyrins comprise a family of tyrosine-phosphorylated proteins with three isoforms: two neuronal forms (synaptogyrin-1 and -3) and one ubiquitous form (synaptogyrin-2). The synaptogyrin 1 (SYNGR1) can act as a regulator of Ca2+-dependent exocytosis. SYNGR1 protein mutations are associated with schizophrenia and are considered to be positional candidate genes for schizophrenia.(PMID: 10383386; 10595519; 14755516; 14732601; 17049558)

实验方案

Product Specific Protocols
WB protocol for SYNGR1 antibody 29734-1-APDownload protocol
IHC protocol for SYNGR1 antibody 29734-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols