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验证数据展示

  • WB analysis using 84452-1-RR

    WB analysis using 84452-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 84452-1-RR

    IHC staining of mouse brain using 84452-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 84452-1-RR

    IHC staining of mouse brain using 84452-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84452-1-RR (Synaptotagmin-5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 84452-1-RR

    IF Staining of mouse brain using 84452-1-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Synaptotagmin-5 antibody (84452-1-RR, Clone: 241452E3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84452-1-RR

    Affinity and Kinetic Characterization of 84452-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84452-1-RR against Human Synaptotagmin-5 were performed. The affinity constant is 0.131 nM.

Synaptotagmin-5 Recombinant antibody

Synaptotagmin-5 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 84452-1-RR

产品说明书

CloneNo. 241452E3

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

SytV, SYT5, Synaptotagmin V, 241452E3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse kidney tissue, rat kidney tissue, human kidney tissue, HUVEC cells, SW 1990 cells, BxPC-3 cells
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84452-1-RR targets Synaptotagmin-5 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Synaptotagmin-5 fusion protein Ag12677 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 synaptotagmin V
别名 SytV, SYT5, Synaptotagmin V, 241452E3
计算分子量 43 kDa
观测分子量42 kDa
GenBank蛋白编号BC046157
基因名称 Synaptotagmin-5
Gene ID (NCBI) 6861
RRIDAB_3671978
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO00445
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). Synaptotagmin-5 (SYT5) belongs to the synaptotagmin family. Synaptotagmin-5 can function as an exocytosis regulator (PMID: 27793683) and is a protein potentially associated with the pathology of schizophrenia (PMID: 32860155).

实验方案

Product Specific Protocols
WB protocol for Synaptotagmin-5 antibody 84452-1-RRDownload protocol
IHC protocol for Synaptotagmin-5 antibody 84452-1-RRDownload protocol
IF protocol for Synaptotagmin-5 antibody 84452-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols