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Syntaxin 10 Monoclonal antibody

Syntaxin 10 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B1C3

Cat no : 66821-1-Ig

Print datasheet

Synonyms

hsyn10, STX10, SYN10, syntaxin 10

验证数据展示

  • WB analysis using 66821-1-Ig

    WB analysis using 66821-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66821-1-Ig (Syntaxin 10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 66821-1-Ig

    IF Staining of HeLa using 66821-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Syntaxin 10 antibody (66821-1-Ig, Clone: 1B1C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, T-47D cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy STX10 Ready-To-Use IHC Kit. STX10 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66821-1-Ig targets Syntaxin 10 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen Syntaxin 10 fusion protein Ag27861 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name syntaxin 10
Synonyms hsyn10, STX10, SYN10, syntaxin 10
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC017237
Gene Symbol Syntaxin 10
Gene ID (NCBI) 8677
RRIDAB_2882164
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO60499
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Syntaxin 10 (STX10), also named as SYN10, belongs to the syntaxin family. Syntaxins are target membrane SNAREs(Soluble NSF attachment protein receptors) which are membrane components that determine the specificity of the docking and fusion of vesicles to target membranes.

实验方案

Product Specific Protocols
WB protocol for Syntaxin 10 antibody 66821-1-IgDownload protocol
IF protocol for Syntaxin 10 antibody 66821-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols