• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

Syntaxin 12 Polyclonal antibody, PBS Only

Syntaxin 12 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14259-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 14259-1-AP (same clone as 14259-1-PBS)

    WB analysis using 14259-1-AP (same clone as 14259-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 14259-1-AP (same clone as 14259-1-PBS)

    IF Staining of HepG2 using 14259-1-AP (same clone as 14259-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using STX12 antibody 14259-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 14259-1-AP (same clone as 14259-1-PBS)

    IHC staining of human lung cancer using 14259-1-AP (same clone as 14259-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 14259-1-AP (same clone as 14259-1-PBS)

    IHC staining of human lung cancer using 14259-1-AP (same clone as 14259-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

产品信息

14259-1-PBS targets Syntaxin 12 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Syntaxin 12 fusion protein Ag5532 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name syntaxin 12
Synonyms
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 33-39 kDa, 66 kDa
GenBank Accession NumberBC046999
Gene Symbol Syntaxin 12
Gene ID (NCBI) 23673
RRIDAB_2198222
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86Y82
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Syntaxin 12 (STX12), also known as STX13 or STX14, is a 276 amino acid single-pass type IV membrane protein that belongs to the syntaxin family. It is a broadly and differentially expressed SNARE protein, highly enriched in brain tissue. STX12 is localized to early and recycling endosomes and is involved in the recycling of plasma membrane proteins. It also has a role in the trafficking of MMP during the degradation of ECM substrates and subsequent cellular invasion. (PMID: 9507000; 9553086; 19910495) This antibody recognizes a major 39 kDa band of endogenous STX12. In some cases, two additional bands of 33 kDa and 66 kDa could also be detected, which most likely represent a degradation product and a SNARE complex respectively (PMID: 9817754).