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验证数据展示

  • WB analysis using 14259-1-AP (same clone as 14259-1-PBS)

    WB analysis using 14259-1-AP (same clone as 14259-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 14259-1-AP (same clone as 14259-1-PBS)

    IF Staining of HepG2 using 14259-1-AP (same clone as 14259-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using STX12 antibody 14259-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 14259-1-AP (same clone as 14259-1-PBS)

    IHC staining of human lung cancer using 14259-1-AP (same clone as 14259-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 14259-1-AP (same clone as 14259-1-PBS)

    IHC staining of human lung cancer using 14259-1-AP (same clone as 14259-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14259-1-AP (Syntaxin 12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14259-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Syntaxin 12 Polyclonal antibody, PBS Only

Syntaxin 12 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14259-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Syntaxin-12, STX12

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

14259-1-PBS targets Syntaxin 12 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Syntaxin 12 fusion protein Ag5532 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 syntaxin 12
别名 Syntaxin-12, STX12
计算分子量 32 kDa
观测分子量 33-39 kDa, 66 kDa
GenBank蛋白编号BC046999
基因名称 Syntaxin 12
Gene ID (NCBI) 23673
RRIDAB_2198222
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86Y82
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Syntaxin 12 (STX12), also known as STX13 or STX14, is a 276 amino acid single-pass type IV membrane protein that belongs to the syntaxin family. It is a broadly and differentially expressed SNARE protein, highly enriched in brain tissue. STX12 is localized to early and recycling endosomes and is involved in the recycling of plasma membrane proteins. It also has a role in the trafficking of MMP during the degradation of ECM substrates and subsequent cellular invasion. (PMID: 9507000; 9553086; 19910495) This antibody recognizes a major 39 kDa band of endogenous STX12. In some cases, two additional bands of 33 kDa and 66 kDa could also be detected, which most likely represent a degradation product and a SNARE complex respectively (PMID: 9817754).