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  • Featured Product
  • KD/KO Validated

Syntaxin 1A / Syntaxin 1B Monoclonal antibody, PBS Only

Syntaxin 1A / Syntaxin 1B Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D3B5

Cat no : 66437-1-PBS

Print datasheet

Synonyms

STX1B, STX1B1, STX1B2, Syntaxin 1A / Syntaxin 1B, syntaxin 1B, Syntaxin 1B1, Syntaxin 1B2

验证数据展示

  • WB analysis using 66437-1-Ig (same clone as 66437-1-PBS)

    WB analysis using 66437-1-Ig (same clone as 66437-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66437-1-Ig (Syntaxin 1A / Syntaxin 1B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 66437-1-Ig (same clone as 66437-1-PBS)

    IHC staining of rat brain using 66437-1-Ig (same clone as 66437-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66437-1-Ig (Syntaxin 1A / Syntaxin 1B antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Syntaxin 1A/1B antibody included
  • IHC staining of rat brain using 66437-1-Ig (same clone as 66437-1-PBS)

    IHC staining of rat brain using 66437-1-Ig (same clone as 66437-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66437-1-Ig (Syntaxin 1A / Syntaxin 1B antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Syntaxin 1A/1B antibody included
  • IF Staining of mouse brain using 66437-1-Ig (same clone as 66437-1-PBS)

    IF Staining of mouse brain using 66437-1-Ig (same clone as 66437-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Syntaxin 1A / Syntaxin 1B antibody (66437-1-Ig, Clone: 2D3B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66437-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy Syntaxin 1A/1B Ready-To-Use IHC Kit. Syntaxin 1A/1B primary antibody included.

产品信息

66437-1-PBS targets Syntaxin 1A / Syntaxin 1B in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, rabbit, pig samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, rabbit, pig
Immunogen Syntaxin 1A / Syntaxin 1B fusion protein Ag10257 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name syntaxin 1B
Synonyms STX1B, STX1B1, STX1B2, Syntaxin 1A / Syntaxin 1B, syntaxin 1B, Syntaxin 1B1, Syntaxin 1B2
Calculated Molecular Weight 288 aa, 33 kDa
Observed Molecular Weight 33 kDa, 55 kDa
GenBank Accession NumberBC062298
Gene Symbol STX1B
Gene ID (NCBI) 112755
RRIDAB_2881807
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP61266
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Syntaxins are a family of transmembrane proteins that belong to the SNARE complex (PMID: 11737951). In conjunction with other SNAREs and with the cytoplasmic NSF and SNAP proteins, syntaxins mediate vesicle fusion in diverse vesicular transport processes along the exocytic and the endocytic pathway (PMID: 11737951). Syntaxin 1A and 1B, two closely related isoforms of syntaxin 1, are involved in synaptic vesicle docking and fusion during neurotransmitter release (PMID: 7690687; 1321498; 18691641). This antibody raised against 1-263 aa of human syntaxin 1B detects a ~33 kDa band of syntaxin 1 and an additional band of 55-60 kDa, which probably represents a syntaxin 1 dimer (PMID: 10100611).