T-47D whole-cell extract

200 µg of T-47D whole-cell extract at 5 µg/µl.

T-47D whole-cell extract was prepared from cell cultures of the human ductal breast epithelial tumor cell line. The T-47D cell line was derived from a pleural effusion of a 54-year old female patient with infiltrating ductal carcinoma. These cells exhibit a differentiated epithelial cell type and express receptors to estradiol, as well as other steroids, and calcitonin. These cells also express mutant tumor suppressor p53 and are therefore commonly used as in vitro models of mutant p53 breast cancer cells to study breast cancer tumorigenicity and related drug therapies, such as tamoxifen.

T-47D whole-cell extract is specifically recommended for use in studies related to breast cancer and associated drug therapies.

Human breast ductal carcinoma

T-47D whole-cell extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl₂, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).

Extracts have been quality control tested by Western blot.