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T7-tag Polyclonal antibody

T7-tag Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 14441-1-AP

Print datasheet

Synonyms

T7 tag, T7-tag

验证数据展示

  • WB analysis using 14441-1-AP

    WB analysis using 14441-1-AP

    T7-tagged fution protein(20ng) was subjected to SDS PAGE followed by western blot with 14441-1-AP (T7-tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inRecombinant protein protein

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14441-1-AP targets T7-tag in WB, ELISA applications and shows reactivity with recombinant protein samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity recombinant protein
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name T7-tag
Synonyms T7 tag, T7-tag
Calculated Molecular Weight 1 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_2878060
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The antibody is developed against a synthetic peptide MASMTGGQQMG, the T7 tag, which is present in many vectors.

实验方案

Product Specific Protocols
WB protocol for T7-tag antibody 14441-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols