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  • Featured Product
  • KD/KO Validated

TAB1 Polyclonal antibody

TAB1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 27566-1-AP

Print datasheet

Synonyms

MAP3K7IP1, TGF-beta-activated kinase 1-binding protein 1, TGF-beta-activated kinase 1 and MAP3K7-binding protein 1, TAK1-binding protein 1, TAK1 binding protein 1

验证数据展示

  • WB analysis using 27566-1-AP

    WB analysis using 27566-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27566-1-AP (TAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 27566-1-AP

    IHC staining of human colon cancer using 27566-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27566-1-AP (TAB1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 27566-1-AP

    IHC staining of human liver using 27566-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27566-1-AP (TAB1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 27566-1-AP

    IHC staining of human liver using 27566-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27566-1-AP (TAB1 antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, A375 cells, K-562 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27566-1-AP targets TAB1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen TAB1 fusion protein Ag25576 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitogen-activated protein kinase kinase kinase 7 interacting protein 1
Synonyms MAP3K7IP1, TGF-beta-activated kinase 1-binding protein 1, TGF-beta-activated kinase 1 and MAP3K7-binding protein 1, TAK1-binding protein 1, TAK1 binding protein 1
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC050554
Gene Symbol TAB1
Gene ID (NCBI) 10454
RRIDAB_2880910
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15750
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TAB1 antibody 27566-1-APDownload protocol
IHC protocol for TAB1 antibody 27566-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Theranostics

Virus-induced p38 MAPK activation facilitates viral infection.

Authors - Yuting Cheng
  • KO Validated
View Article
humanWB,IF

Int J Mol Sci

MicroRNA-27a-5p Downregulates Expression of Proinflammatory Cytokines in Lipopolysaccharide-Stimulated Human Dental Pulp Cells via the NF-κB Signaling Pathway

Authors - Shihan Wang
  • KD Validated
View Article