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验证数据展示

  • WB analysis using 27566-1-AP

    WB analysis using 27566-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27566-1-AP (TAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 27566-1-AP

    IHC staining of human colon cancer using 27566-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27566-1-AP (TAB1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 27566-1-AP

    IHC staining of human liver using 27566-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27566-1-AP (TAB1 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 27566-1-AP

    IHC staining of human liver using 27566-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27566-1-AP (TAB1 antibody) at dilution of 1:200 (under 40x lens).

  • Featured Product
  • KD/KO验证

TAB1 Polyclonal antibody

TAB1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27566-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA and More (1)

别名

MAP3K7IP1, TGF-beta-activated kinase 1-binding protein 1, TGF-beta-activated kinase 1 and MAP3K7-binding protein 1, TAK1-binding protein 1, TAK1 binding protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, A375 cells, K-562 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27566-1-AP targets TAB1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse
文献引用反应性human
免疫原 TAB1 fusion protein Ag25576 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitogen-activated protein kinase kinase kinase 7 interacting protein 1
别名 MAP3K7IP1, TGF-beta-activated kinase 1-binding protein 1, TGF-beta-activated kinase 1 and MAP3K7-binding protein 1, TAK1-binding protein 1, TAK1 binding protein 1
计算分子量 55 kDa
观测分子量 55 kDa
GenBank蛋白编号BC050554
基因名称 TAB1
Gene ID (NCBI) 10454
RRIDAB_2880910
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15750
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TAB1 antibody 27566-1-APDownload protocol
IHC protocol for TAB1 antibody 27566-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Theranostics

Virus-induced p38 MAPK activation facilitates viral infection.

Authors - Yuting Cheng
  • KO Validated
View Article
humanWB,IF

Int J Mol Sci

MicroRNA-27a-5p Downregulates Expression of Proinflammatory Cytokines in Lipopolysaccharide-Stimulated Human Dental Pulp Cells via the NF-κB Signaling Pathway

Authors - Shihan Wang
  • KD Validated
View Article