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TAB1 Monoclonal antibody

TAB1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1E10D12

Cat no : 67020-1-Ig

Print datasheet

Synonyms

MAP3K7IP1, TGF-beta-activated kinase 1-binding protein 1, TGF-beta-activated kinase 1 and MAP3K7-binding protein 1, TAK1-binding protein 1, TAK1 binding protein 1

验证数据展示

  • WB analysis of HEK-293 using 67020-1-Ig

    WB analysis of HEK-293 using 67020-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67020-1-Ig (TAB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 67020-1-Ig

    IHC staining of human lung cancer using 67020-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67020-1-Ig (TAB1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 67020-1-Ig

    IHC staining of human lung cancer using 67020-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67020-1-Ig (TAB1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 67020-1-Ig

    IF Staining of A431 using 67020-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using TAB1 antibody (67020-1-Ig, Clone: 1E10D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HSC-T6 cells, K-562 cells, MCF-7 cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67020-1-Ig targets TAB1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen TAB1 fusion protein Ag6590 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name mitogen-activated protein kinase kinase kinase 7 interacting protein 1
Synonyms MAP3K7IP1, TGF-beta-activated kinase 1-binding protein 1, TGF-beta-activated kinase 1 and MAP3K7-binding protein 1, TAK1-binding protein 1, TAK1 binding protein 1
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC050554
Gene Symbol TAB1
Gene ID (NCBI) 10454
RRIDAB_2882336
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15750
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TAB1 antibody 67020-1-IgDownload protocol
IHC protocol for TAB1 antibody 67020-1-IgDownload protocol
IF protocol for TAB1 antibody 67020-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Hum Mutat

The MAP3K7 gene: further delineation of clinical characteristics and genotype/phenotype correlations.

Authors - Geeske M van Woerden
View Article
humanWB,IP

Leukemia

Thrombin receptor activating peptide-6 decreases acute graft-versus-host disease through activating GPR15

Authors - Cong Liu
View Article