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验证数据展示

  • IHC staining of mouse brain using 28599-1-AP

    IHC staining of mouse brain using 28599-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28599-1-AP (TAC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TAC1 antibody included
  • IHC staining of mouse brain using 28599-1-AP

    IHC staining of mouse brain using 28599-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28599-1-AP (TAC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TAC1 antibody included
  • IF Staining of mouse brain using 28599-1-AP

    IF Staining of mouse brain using 28599-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TAC1 antibody (28599-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse brain using 28599-1-AP

    IF Staining of mouse brain using 28599-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TAC1 antibody (28599-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of PC-12 using 28599-1-AP

    IF Staining of PC-12 using 28599-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using TAC1 antibody (28599-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

TAC1 Polyclonal antibody

TAC1 Polyclonal Antibody for IHC, IF/ICC, IF-P, ELISA
Cat No. 28599-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, IF-P, ELISA

别名

TACI, TAC 1, Protachykinin-1, Protachykinin 1, C-terminal-flanking peptide

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse brain tissue, human hypothalamus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inPC-12 cells
Planning an IHC experiment? We recommend our IHCeasy TAC1 Ready-To-Use IHC Kit. TAC1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28599-1-AP targets TAC1 in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TAC1 fusion protein Ag23477 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tachykinin, precursor 1
别名 TACI, TAC 1, Protachykinin-1, Protachykinin 1, C-terminal-flanking peptide
计算分子量 129 aa, 15 kDa
GenBank蛋白编号BC018047
基因名称 TAC1
Gene ID (NCBI) 6863
RRIDAB_2881178
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP20366
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TAC1, also known as Protachykinin-1, is a gene that encodes for a family of neuropeptides known as tachykinins. These peptides are characterized by their ability to rapidly stimulate contraction of intestinal muscle, hence the name "tachykinins". The TAC1 gene and its products are widely distributed in the nervous system, with α-TAC1 mRNA localized in numerous neurons of the brain. β-TAC1 and γ-TAC1 are predominantly expressed in intrinsic enteric neurons and sensory neurons. Additionally, the expression of TAC1, SP, and NKA is observed in various peripheral neurons and non-neural tissues such as the salivary gland, heart, skin, spleen, adrenal gland, and more.

实验方案

Product Specific Protocols
IHC protocol for TAC1 antibody 28599-1-APDownload protocol
IF protocol for TAC1 antibody 28599-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols