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TACC1 Polyclonal antibody

TACC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 13862-1-AP

Print datasheet

Synonyms

Transforming acidic coiled-coil-containing protein 1, Taxin-1, Taxin1, KIAA1103, Ga55

验证数据展示

  • WB analysis of MCF-7 using 13862-1-AP

    WB analysis of MCF-7 using 13862-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 13862-1-AP (TACC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 13862-1-AP

    IHC staining of human stomach cancer using 13862-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13862-1-AP (TACC1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TACC1 antibody included
  • IHC staining of human stomach cancer using 13862-1-AP

    IHC staining of human stomach cancer using 13862-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13862-1-AP (TACC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TACC1 antibody included
  • IF Staining of HeLa using 13862-1-AP

    IF Staining of HeLa using 13862-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TACC1 antibody (13862-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inMCF7 cells, HeLa cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TACC1 Ready-To-Use IHC Kit. TACC1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

13862-1-AP targets TACC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human
Cited Reactivityhuman
Immunogen TACC1 fusion protein Ag4842 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transforming, acidic coiled-coil containing protein 1
Synonyms Transforming acidic coiled-coil-containing protein 1, Taxin-1, Taxin1, KIAA1103, Ga55
Calculated Molecular Weight 805 aa, 88 kDa
Observed Molecular Weight 110-130 kDa
GenBank Accession NumberBC041391
Gene Symbol TACC1
Gene ID (NCBI) 6867
RRIDAB_2255719
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75410
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TACC1 antibody 13862-1-APDownload protocol
IHC protocol for TACC1 antibody 13862-1-APDownload protocol
IF protocol for TACC1 antibody 13862-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cell Death Discov

Loss of TACC1 variant25 inducing cell proliferation and suppressing autophagy in head and neck squamous carcinoma.

Authors - Pan Xu
View Article