验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
11407-1-AP targets TACC2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | TACC2 fusion protein Ag1923 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | transforming, acidic coiled-coil containing protein 2 |
| Synonyms | Anti Zuai 1, AZU 1, ECTACC, TACC2 |
| Calculated Molecular Weight | 2948 aa, 309 kDa |
| Observed Molecular Weight | 73 kDa |
| GenBank Accession Number | BC015736 |
| Gene Symbol | TACC2 |
| Gene ID (NCBI) | 10579 |
| RRID | AB_2200456 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95359 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Transforming acidic coiled-coil (TACC) proteins are a conserved family of centrosome- and microtubule-interacting proteins that are implicated in cancer. TACC2, also known as anti-zuai-1 (AZU-1), was abundantly expressed in non- and premalignant cells and tissues but was appreciably reduced in breast tumor cell types and in primary tumors, thus TACC2 may act as a tumor suppressor protein and represent a tumor progression marker. Several transcript variants encoding different isoforms have been found for this gene. Chromosome segregation in mitosis is orchestrated by dynamic interaction between spindle microtubule and the kinetochore. Centrosomal TACC2 plays a role in organizing centrosomal microtubules and is required for mitotic spindle maintenance.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TACC2 antibody 11407-1-AP | Download protocol |
| IHC protocol for TACC2 antibody 11407-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Biomark High expression of TACC2 in hepatocellular carcinoma is associated with poor prognosis. | ||
F1000Res Androgen-dependent alternative mRNA isoform expression in prostate cancer cells. |