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验证数据展示

  • WB analysis using 29856-1-AP

    WB analysis using 29856-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29856-1-AP (TACSTD2/TROP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HaCaT using 29856-1-AP

    IF Staining of HaCaT using 29856-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using TACSTD2/TROP2 antibody (29856-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human ovary cancer using 29856-1-AP

    IHC staining of human ovary cancer using 29856-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29856-1-AP (TACSTD2/TROP2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TACSTD2 antibody included
  • IHC staining of human skin cancer using 29856-1-AP

    IHC staining of human skin cancer using 29856-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 29856-1-AP (TACSTD2/TROP2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TACSTD2 antibody included
  • IHC staining of human intrahepatic cholangiocarcinoma using 29856-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 29856-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 29856-1-AP (TACSTD2/TROP2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TACSTD2 antibody included

TACSTD2/TROP2 Polyclonal antibody

TACSTD2/TROP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 29856-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

TACSTD2, M1S1, GA733-1, GA733 1, GA733

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, HaCaT cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHaCaT cells
Planning an IHC experiment? We recommend our IHCeasy TACSTD2 Ready-To-Use IHC Kit. TACSTD2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29856-1-AP targets TACSTD2/TROP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 TACSTD2/TROP2 fusion protein Ag31473 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tumor-associated calcium signal transducer 2
别名 TACSTD2, M1S1, GA733-1, GA733 1, GA733
计算分子量 36 kDa
观测分子量45-50 kDa
GenBank蛋白编号BC009409
基因名称 TROP2
Gene ID (NCBI) 4070
RRIDAB_3086179
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP09758
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Trophoblast cell surface antigen 2 (TROP2), also named as TACSTD2, is a transmembrane glycoprotein. TROP2 plays a role in transducing intracellular calcium signals. It is expressed in trophoblast cells, cornea and multi-stratified epithelia. TROP2 is overexpressed in most carcinomas and is involved in cancer proliferation, migration, invasion, and metastasis. Congenital mutations of TROP2 cause a rare autosomal recessive disease which may lead to blindness-the gelatinous drop-like corneal disease (PMID: 35688908).

实验方案

Product Specific Protocols
WB protocol for TACSTD2/TROP2 antibody 29856-1-APDownload protocol
IHC protocol for TACSTD2/TROP2 antibody 29856-1-APDownload protocol
IF protocol for TACSTD2/TROP2 antibody 29856-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols