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TACSTD2/TROP2 Polyclonal antibody

TACSTD2/TROP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29856-1-AP

Print datasheet

Synonyms

TACSTD2, M1S1, GA733-1, GA733 1, GA733

验证数据展示

  • WB analysis using 29856-1-AP

    WB analysis using 29856-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29856-1-AP (TACSTD2/TROP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HaCaT using 29856-1-AP

    IF Staining of HaCaT using 29856-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using TACSTD2/TROP2 antibody (29856-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human ovary cancer using 29856-1-AP

    IHC staining of human ovary cancer using 29856-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29856-1-AP (TACSTD2/TROP2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TACSTD2 antibody included
  • IHC staining of human skin cancer using 29856-1-AP

    IHC staining of human skin cancer using 29856-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 29856-1-AP (TACSTD2/TROP2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TACSTD2 antibody included
  • IHC staining of human intrahepatic cholangiocarcinoma using 29856-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 29856-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 29856-1-AP (TACSTD2/TROP2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TACSTD2 antibody included

经过测试的应用

Positive WB detected inA431 cells, HaCaT cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHaCaT cells
Planning an IHC experiment? We recommend our IHCeasy TACSTD2 Ready-To-Use IHC Kit. TACSTD2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29856-1-AP targets TACSTD2/TROP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen TACSTD2/TROP2 fusion protein Ag31473 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tumor-associated calcium signal transducer 2
Synonyms TACSTD2, M1S1, GA733-1, GA733 1, GA733
Calculated Molecular Weight 36 kDa
Observed Molecular Weight45-50 kDa
GenBank Accession NumberBC009409
Gene Symbol TROP2
Gene ID (NCBI) 4070
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09758
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Trophoblast cell surface antigen 2 (TROP2), also named as TACSTD2, is a transmembrane glycoprotein. TROP2 plays a role in transducing intracellular calcium signals. It is expressed in trophoblast cells, cornea and multi-stratified epithelia. TROP2 is overexpressed in most carcinomas and is involved in cancer proliferation, migration, invasion, and metastasis. Congenital mutations of TROP2 cause a rare autosomal recessive disease which may lead to blindness-the gelatinous drop-like corneal disease (PMID: 35688908).

实验方案

Product Specific Protocols
WB protocol for TACSTD2/TROP2 antibody 29856-1-APDownload protocol
IHC protocol for TACSTD2/TROP2 antibody 29856-1-APDownload protocol
IF protocol for TACSTD2/TROP2 antibody 29856-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols