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TADA3L Polyclonal antibody

TADA3L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10839-1-AP

Print datasheet

Synonyms

ADA3, ADA3 homolog, ADA3 like protein, hADA3, STAF54, TADA3, TADA3L, Transcriptional adapter 3, Transcriptional adapter 3 like

验证数据展示

  • WB analysis using 10839-1-AP

    WB analysis using 10839-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10839-1-AP (TADA3L antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 10839-1-AP

    IHC staining of human prostate cancer using 10839-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10839-1-AP (TADA3L antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with TADA3L antibody included

经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells, human brain tissue, Jurkat cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TADA3L Ready-To-Use IHC Kit. TADA3L primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:9000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10839-1-AP targets TADA3L in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen TADA3L fusion protein Ag1276 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transcriptional adaptor 3 (NGG1 homolog, yeast)-like
Synonyms ADA3, ADA3 homolog, ADA3 like protein, hADA3, STAF54, TADA3, TADA3L, Transcriptional adapter 3, Transcriptional adapter 3 like
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC009240
Gene Symbol TADA3L
Gene ID (NCBI) 10474
RRIDAB_513667
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75528
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TADA3 is a subunit of 2 histone acetyltransferase complexes: the ADA2A (TADA2A)-containing (ATAC) complex and the SPT3 (SUPT3H)-TAF9-GCN5 (KAT2A)/PCAF (KAT2B) acetylase (STAGA) complex. The ATAC complex is a complex with histone acetyltransferase activity on histones H3 and H4, and the PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. TADA3 is also known as a coactivator for p53/TP53-dependent transcriptional activation.

实验方案

Product Specific Protocols
WB protocol for TADA3L antibody 10839-1-APDownload protocol
IHC protocol for TADA3L antibody 10839-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Improved in situ characterization of protein complex dynamics at scale with thermal proximity co-aggregation

Authors - Siyuan Sun
View Article