验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, NIH/3T3 cells, human brain tissue, Jurkat cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy TADA3L Ready-To-Use IHC Kit. TADA3L primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:9000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
10839-1-AP targets TADA3L in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | TADA3L fusion protein Ag1276 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transcriptional adaptor 3 (NGG1 homolog, yeast)-like |
| 别名 | ADA3, ADA3 homolog, ADA3 like protein, hADA3, STAF54, TADA3, TADA3L, Transcriptional adapter 3, Transcriptional adapter 3 like |
| 计算分子量 | 41 kDa |
| 观测分子量 | 49 kDa |
| GenBank蛋白编号 | BC009240 |
| 基因名称 | TADA3L |
| Gene ID (NCBI) | 10474 |
| RRID | AB_513667 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75528 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TADA3 is a subunit of 2 histone acetyltransferase complexes: the ADA2A (TADA2A)-containing (ATAC) complex and the SPT3 (SUPT3H)-TAF9-GCN5 (KAT2A)/PCAF (KAT2B) acetylase (STAGA) complex. The ATAC complex is a complex with histone acetyltransferase activity on histones H3 and H4, and the PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. TADA3 is also known as a coactivator for p53/TP53-dependent transcriptional activation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TADA3L antibody 10839-1-AP | Download protocol |
| IHC protocol for TADA3L antibody 10839-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
