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TAF10 Polyclonal antibody

TAF10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20346-1-AP

Print datasheet

Synonyms

STAF28, TAF(II)30, TAF10, TAF2A, TAF2H, TAFII 30, TAFII30

验证数据展示

  • WB analysis of mouse testis using 20346-1-AP

    WB analysis of mouse testis using 20346-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20346-1-AP (TAF10 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 20346-1-AP

    IHC staining of mouse testis using 20346-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20346-1-AP (TAF10 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:500
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 20346-1-AP is TAF10 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen TAF10 fusion protein Ag14153 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TAF10 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 30kDa
Synonyms STAF28, TAF(II)30, TAF10, TAF2A, TAF2H, TAFII 30, TAFII30
Calculated Molecular Weight 218 aa, 22 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC012088
Gene Symbol TAF10
Gene ID (NCBI) 6881
RRIDAB_2878678
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12962
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TAF10 antibody 20346-1-APDownload protocol
IHC protocol for TAF10 antibody 20346-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochem Pharmacol

Calcium saccharate/DUSP6 suppresses renal cell carcinoma glycolytic metabolism and boosts sunitinib efficacy via the ERK-AKT pathway

Authors - Huan Liu
View Article