验证数据展示
经过测试的应用
| Positive WB detected in | Y79 cells |
| Positive IP detected in | Y79 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12353-1-AP targets TAF12 in WB, IHC, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, ChIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | TAF12 fusion protein Ag3017 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa |
| 别名 | TAF12, TAF15, TAF2J, TAFII 20/TAFII 15, TAFII20, TAFII20/TAFII15 |
| 计算分子量 | 18 kDa |
| 观测分子量 | 21 kDa |
| GenBank蛋白编号 | BC011986 |
| 基因名称 | TAF12 |
| Gene ID (NCBI) | 6883 |
| RRID | AB_2271582 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16514 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TAF12, also named TAF15 or TAF2J, is a TBP-associated factor that is contained in Pol I- and Pol II-specific TBP-TAF complexes, it is also a component of the transcription factor IID (TFIID) complex, which is essential for mediating regulation of RNA polymerase transcription. TAF12 plays a key role in regulating eukaryotic gene expression by directly binding promoters and enhancer-bound transactivator proteins. Likewise, TAF12 recruits Gadd45a and the nucleotide excision repair complex to the promoter of rRNA genes leading to active DNA demethylation. Observed MW of TAF12 is from 14-17 kDa and 17-21 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TAF12 antibody 12353-1-AP | Download protocol |
| IP protocol for TAF12 antibody 12353-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Cell A TFIID-SAGA Perturbation that Targets MYB and Suppresses Acute Myeloid Leukemia.
| ||
Cell Metab Contributions of the measles virus nucleocapsid gene and the SQSTM1/p62(P392L) mutation to Paget's disease. | ||
J Bone Miner Res Role of ATF7-TAF12 interactions in the vitamin D response hypersensitivity of osteoclast precursors in Paget's disease. | ||
J Bone Miner Res TBK1 mediates critical effects of measles virus nucleocapsid protein (MVNP) on pagetic osteoclast formation. | ||
J Bone Miner Res Increased IL-6 expression in osteoclasts is necessary but not sufficient for the development of Paget's disease of bone. | ||
Mol Cell Biol The general transcription factor TAF7 is essential for embryonic development but not essential for the survival or differentiation of mature T cells. |