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  • Featured Product
  • KD/KO Validated

TAF15 Polyclonal antibody

TAF15 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25521-1-AP

Print datasheet

Synonyms

TAF(II)68, RNA-binding protein 56, RNA binding protein 56, RBP56, hTAFII68

验证数据展示

  • WB analysis of Jurkat using 25521-1-AP

    WB analysis of Jurkat using 25521-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25521-1-AP (TAF15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 25521-1-AP

    WB analysis of HEK-293 using 25521-1-AP

    WB result of TAF15 antibody (25521-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAF15 transfected HEK-293 cells.

  • IHC staining of human colon cancer using 25521-1-AP

    IHC staining of human colon cancer using 25521-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25521-1-AP (TAF15 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TAF15 antibody included
  • IHC staining of human colon cancer using 25521-1-AP

    IHC staining of human colon cancer using 25521-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25521-1-AP (TAF15 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TAF15 antibody included
  • IHC staining of human malignant melanoma using 25521-1-AP

    IHC staining of human malignant melanoma using 25521-1-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 25521-1-AP (TAF15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TAF15 antibody included
  • IHC staining of human cervical cancer using 25521-1-AP

    IHC staining of human cervical cancer using 25521-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25521-1-AP (TAF15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TAF15 antibody included

经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells, mouse brain tissue, SH-SY5Y cells, A549 cells
Positive IHC detected inhuman colon cancer tissue, human cervical cancer tissue, human kidney tissue, human malignant melanoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TAF15 Ready-To-Use IHC Kit. TAF15 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25521-1-AP targets TAF15 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa
Synonyms TAF(II)68, RNA-binding protein 56, RNA binding protein 56, RBP56, hTAFII68
Calculated Molecular Weight 62 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberNM_003487
Gene Symbol TAF15
Gene ID (NCBI) 8148
RRIDAB_2880116
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92804
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TAF15 antibody 25521-1-APDownload protocol
IHC protocol for TAF15 antibody 25521-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols