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验证数据展示

  • WB analysis of Jurkat using 25521-1-AP

    WB analysis of Jurkat using 25521-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25521-1-AP (TAF15 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 25521-1-AP

    WB analysis of HEK-293 using 25521-1-AP

    WB result of TAF15 antibody (25521-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAF15 transfected HEK-293 cells.

  • IHC staining of human colon cancer using 25521-1-AP

    IHC staining of human colon cancer using 25521-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25521-1-AP (TAF15 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TAF15 antibody included
  • IHC staining of human colon cancer using 25521-1-AP

    IHC staining of human colon cancer using 25521-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25521-1-AP (TAF15 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TAF15 antibody included
  • IHC staining of human malignant melanoma using 25521-1-AP

    IHC staining of human malignant melanoma using 25521-1-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 25521-1-AP (TAF15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TAF15 antibody included
  • IHC staining of human cervical cancer using 25521-1-AP

    IHC staining of human cervical cancer using 25521-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 25521-1-AP (TAF15 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TAF15 antibody included
  • Featured Product
  • KD/KO验证

TAF15 Polyclonal antibody

TAF15 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25521-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

TAF(II)68, RNA-binding protein 56, RNA binding protein 56, RBP56, hTAFII68

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells, mouse brain tissue, SH-SY5Y cells, A549 cells
Positive IHC detected inhuman colon cancer tissue, human cervical cancer tissue, human kidney tissue, human malignant melanoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TAF15 Ready-To-Use IHC Kit. TAF15 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25521-1-AP targets TAF15 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa
别名 TAF(II)68, RNA-binding protein 56, RNA binding protein 56, RBP56, hTAFII68
计算分子量 62 kDa
观测分子量 65-70 kDa
GenBank蛋白编号NM_003487
基因名称 TAF15
Gene ID (NCBI) 8148
RRIDAB_2880116
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92804
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TAF15 antibody 25521-1-APDownload protocol
IHC protocol for TAF15 antibody 25521-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

TFII-I/GTF2I regulates globin gene expression and stress response in erythroid cells

Authors - Rukiye Nar
View Article