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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 82860-1-RR (same clone as 82860-1-PBS)

    WB analysis using 82860-1-RR (same clone as 82860-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82860-1-RR (TAF15 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82860-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 82860-1-RR (same clone as 82860-1-PBS)

    WB analysis of HeLa using 82860-1-RR (same clone as 82860-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82860-1-RR (TAF15 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82860-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 82860-1-RR (same clone as 82860-1-PBS)

    WB analysis of HeLa using 82860-1-RR (same clone as 82860-1-PBS)

    WB result of TAF15 antibody (82860-1-RR; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAF15 transfected HeLa cells. This data was developed using the same antibody clone with 82860-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82860-1-RR (same clone as 82860-1-PBS)

    IF Staining of HeLa using 82860-1-RR (same clone as 82860-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TAF15 antibody (82860-1-RR, Clone: 3L13 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).CoraLite® Plus 488-Phalloidin (green) This data was developed using the same antibody clone with 82860-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82860-1-PBS

    Affinity and Kinetic Characterization of 82860-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 82860-1-RR against Human TAF15 were performed. The affinity constant is 0.974 nM.

TAF15 Recombinant antibody, PBS Only

TAF15 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 82860-1-PBS

产品说明书

CloneNo. 3L13

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

RNA-binding protein 56, RNA binding protein 56, RBP56, hTAFII68, 68 kDa TATA-binding protein-associated factor

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy TAF15 Ready-To-Use IHC Kit. TAF15 primary antibody included.

产品信息

82860-1-PBS targets TAF15 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 TAF15 fusion protein Ag20828 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa
别名 RNA-binding protein 56, RNA binding protein 56, RBP56, hTAFII68, 68 kDa TATA-binding protein-associated factor
计算分子量 592 aa, 62 kDa
观测分子量62-75 kDa
GenBank蛋白编号BC046099
基因名称 TAF15
Gene ID (NCBI) 8148
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92804
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TAF15 (TAF2N or TAFII68) is a nuclear protein known to associate with a distinct subpopulation of transcription factor IID (TFIID), a multi-subunit complex that nucleates the pre-initiation complex on promoters of on protein-coding genes. TAF15 harbors a transcriptional activation domain, a RNA recognition motif and many Arg-Gly-Gly repeats that participate in RNA binding. TAF15 along with FUS and EWS constitutes the FET (FUS/EWS/TAF15) protein family of heterogeneous nuclear ribonucleoproteins (hnRNPs) class of RNA binding proteins that are multifunctional proteins implicated in numerous aspects of RNA processing/functions. TAF15 is a nuclear protein that shuttle between cytoplasm-nucleus and as RNA/ssDNA-binding protein, it play specific roles during transcription initiation at distinct promoters and enter the preinitiation complex together with the RNA polymerase II. TAF15 as well as other FET member genes are frequently translocated in sarcomas and rare hematopoietic as well as epithelial cancers. Moreover, dominant mutations in genes coding for four hnRNPs (TDP-43, FUS/TLS, TAF15, and EWS) are found in familial and sporadic cases of ALS and a chromosomal aberration involving TAF15/TAF2N is found in a form of extraskeletal myxoid chondrosarcomas (EMC).