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验证数据展示

  • WB analysis of HeLa using 12818-1-AP

    WB analysis of HeLa using 12818-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12818-1-AP (TAF1B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 12818-1-AP

    IHC staining of human testis using 12818-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12818-1-AP (TAF1B antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human testis using 12818-1-AP

    IHC staining of human testis using 12818-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12818-1-AP (TAF1B antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis using 12818-1-AP

    WB analysis using 12818-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12818-1-AP (TAF1B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

TAF1B Polyclonal antibody

TAF1B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12818-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

RAF1B, RAFI63, SL1, TAF1B, TAFI63, TBP associated factor 1B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12818-1-AP targets TAF1B in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 TAF1B fusion protein Ag3267 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TATA box binding protein (TBP)-associated factor, RNA polymerase I, B, 63kDa
别名 RAF1B, RAFI63, SL1, TAF1B, TAFI63, TBP associated factor 1B
计算分子量 588 aa, 69 kDa
观测分子量 61 kDa
GenBank蛋白编号BC018137
基因名称 TAF1B
Gene ID (NCBI) 9014
RRIDAB_10644160
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ53T94
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TAF1B antibody 12818-1-APDownload protocol
IHC protocol for TAF1B antibody 12818-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Nat Commun

Genome-wide analyses of non-syndromic cleft lip with palate identify 14 novel loci and genetic heterogeneity.

Authors - Yanqin Yu
View Article