验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, cells, SW480 cells, Y79 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
18901-1-AP targets TAF3 in WB, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | TAF3 fusion protein Ag13536 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | TAF3 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 140kDa |
| Synonyms | TAFII-140, TAFII140, TAFII 140, TAF140, TAF(II)140 |
| Calculated Molecular Weight | 929 aa, 104 kDa |
| Observed Molecular Weight | 140 kDa |
| GenBank Accession Number | BC024661 |
| Gene Symbol | TAF3 |
| Gene ID (NCBI) | 83860 |
| RRID | AB_2878567 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5VWG9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TAF3, also named as TAF140, is a 140 kDa subunit of Transcription initiation factor TFIID. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAF3 has some isoforms with 727aa (~80 kDa), 622aa and 929aa (105 kDa).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TAF3 antibody 18901-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |