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TAF5L Polyclonal antibody

TAF5L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 19274-1-AP

Print datasheet

Synonyms

TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L, PCAF-associated factor 65 beta, PCAF associated factor 65 beta, PAF65-beta, PAF65B

验证数据展示

  • WB analysis of Jurkat using 19274-1-AP

    WB analysis of Jurkat using 19274-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19274-1-AP (TAF5L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 19274-1-AP

    IHC staining of human ovary cancer using 19274-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19274-1-AP (TAF5L antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19274-1-AP targets TAF5L in WB, IHC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, CoIP
Tested Reactivity human
Cited Reactivityhuman
Immunogen TAF5L fusion protein Ag5942 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TAF5-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, 65kDa
Synonyms TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L, PCAF-associated factor 65 beta, PCAF associated factor 65 beta, PAF65-beta, PAF65B
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC041094
Gene Symbol TAF5L
Gene ID (NCBI) 27097
RRIDAB_10642700
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75529
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TAF5L antibody 19274-1-APDownload protocol
IHC protocol for TAF5L antibody 19274-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanCoIP

Nat Struct Mol Biol

A cohesin traffic pattern genetically linked to gene regulation

Authors - Anne-Laure Valton
View Article
humanWB

Sci Adv

The KAT module of the SAGA complex maintains the oncogenic gene expression program in MYCN-amplified neuroblastoma

Authors - Clare F Malone
View Article