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验证数据展示

  • WB analysis of Jurkat using 19274-1-AP

    WB analysis of Jurkat using 19274-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19274-1-AP (TAF5L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 19274-1-AP

    IHC staining of human ovary cancer using 19274-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19274-1-AP (TAF5L antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TAF5L Polyclonal antibody

TAF5L Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19274-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA and More (1)

别名

TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L, PCAF-associated factor 65 beta, PCAF associated factor 65 beta, PAF65-beta, PAF65B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19274-1-AP targets TAF5L in WB, IHC, CoIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, CoIP
经测试反应性 human
文献引用反应性human
免疫原 TAF5L fusion protein Ag5942 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TAF5-like RNA polymerase II, p300/CBP-associated factor (PCAF)-associated factor, 65kDa
别名 TAF5-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 5L, PCAF-associated factor 65 beta, PCAF associated factor 65 beta, PAF65-beta, PAF65B
计算分子量 66 kDa
观测分子量 66 kDa
GenBank蛋白编号BC041094
基因名称 TAF5L
Gene ID (NCBI) 27097
RRIDAB_10642700
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75529
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TAF5L antibody 19274-1-APDownload protocol
IHC protocol for TAF5L antibody 19274-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanCoIP

Nat Struct Mol Biol

A cohesin traffic pattern genetically linked to gene regulation

Authors - Anne-Laure Valton
View Article
humanWB

Sci Adv

The KAT module of the SAGA complex maintains the oncogenic gene expression program in MYCN-amplified neuroblastoma

Authors - Clare F Malone
View Article