验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HeLa cells, MCF-7 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy TAF7 Ready-To-Use IHC Kit. TAF7 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
13506-1-AP targets TAF7 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 | TAF7 fusion protein Ag4406 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 55kDa |
| 别名 | TAF(II)55, TAF2F, TAF7, TAFII 55, TAFII55 |
| 计算分子量 | 349 aa, 40 kDa |
| 观测分子量 | 55 kDa |
| GenBank蛋白编号 | BC032737 |
| 基因名称 | TAF7 |
| Gene ID (NCBI) | 6879 |
| RRID | AB_2271400 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15545 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TAF7, also named as Transcription initiation factor TFIID 55 kDa subunit, is a 349 amino acid protein, which belongs to the TAF7 family. TAF7 functions as a component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAF7 is phosphorylated by CIITA. The calcualted molecular weight of TAF7 is 40 kDa, but phosphorylated TAF7 is about 55kDa. (PMID: 22711989)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TAF7 antibody 13506-1-AP | Download protocol |
| IHC protocol for TAF7 antibody 13506-1-AP | Download protocol |
| IP protocol for TAF7 antibody 13506-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
