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TAF7 Polyclonal antibody

TAF7 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13506-1-AP

Print datasheet

Synonyms

TAF(II)55, TAF2F, TAF7, TAFII 55, TAFII55

验证数据展示

  • WB analysis using 13506-1-AP

    WB analysis using 13506-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13506-1-AP (TAF7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 13506-1-AP

    IHC staining of human cervical cancer using 13506-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13506-1-AP (TAF7 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with TAF7 antibody included
  • IHC staining of human cervical cancer using 13506-1-AP

    IHC staining of human cervical cancer using 13506-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 13506-1-AP (TAF7 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with TAF7 antibody included
  • IP experiment of Jurkat using 13506-1-AP

    IP experiment of Jurkat using 13506-1-AP

    IP result of anti-TAF7 (IP:13506-1-AP, 4ug; Detection:13506-1-AP 1:500) with Jurkat cells lysate 1200ug.

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, MCF-7 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TAF7 Ready-To-Use IHC Kit. TAF7 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13506-1-AP targets TAF7 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TAF7 fusion protein Ag4406 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TAF7 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 55kDa
Synonyms TAF(II)55, TAF2F, TAF7, TAFII 55, TAFII55
Calculated Molecular Weight 349 aa, 40 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC032737
Gene Symbol TAF7
Gene ID (NCBI) 6879
RRIDAB_2271400
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15545
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TAF7, also named as Transcription initiation factor TFIID 55 kDa subunit, is a 349 amino acid protein, which belongs to the TAF7 family. TAF7 functions as a component of the DNA-binding general transcription factor complex TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. TAF7 is phosphorylated by CIITA. The calcualted molecular weight of TAF7 is 40 kDa, but phosphorylated TAF7 is about 55kDa. (PMID: 22711989)

实验方案

Product Specific Protocols
WB protocol for TAF7 antibody 13506-1-APDownload protocol
IHC protocol for TAF7 antibody 13506-1-APDownload protocol
IP protocol for TAF7 antibody 13506-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols