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transgelin/SM22 Recombinant antibody, PBS Only

transgelin/SM22 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240864A11

Cat no : 83922-2-PBS

Print datasheet

Synonyms

SM22, transgelin, SM22-alpha, SM22-a, Protein WS3-10

验证数据展示

  • WB analysis using 83922-2-RR (same clone as 83922-2-PBS)

    WB analysis using 83922-2-RR (same clone as 83922-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83922-2-RR (transgelin/SM22 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83922-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 83922-2-RR (same clone as 83922-2-PBS)

    IHC staining of mouse heart using 83922-2-RR (same clone as 83922-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83922-2-RR (transgelin/SM22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83922-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TAGLN antibody included
  • IHC staining of mouse heart using 83922-2-RR (same clone as 83922-2-PBS)

    IHC staining of mouse heart using 83922-2-RR (same clone as 83922-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83922-2-RR (transgelin/SM22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83922-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TAGLN antibody included
  • Affinity and Kinetic Characterization of 83922-2-RR

    Affinity and Kinetic Characterization of 83922-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83922-2-RR against Human transgelin/SM22 were performed. The affinity constant is 14.2 nM.

Planning an IHC experiment? We recommend our IHCeasy TAGLN Ready-To-Use IHC Kit. TAGLN primary antibody included.

产品信息

The immunogen of 83922-2-PBS is transgelin/SM22 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen transgelin/SM22 fusion protein Ag0764 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name transgelin
Synonyms SM22, transgelin, SM22-alpha, SM22-a, Protein WS3-10
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 19-22 kDa
GenBank Accession NumberBC004927
Gene Symbol transgelin/SM22
Gene ID (NCBI) 6876
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ01995
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The transgelin family is a protein group belonging to the 22kd actin-related calponin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, or SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody was generated against full-length transgelin 1 protein. It can cross-react with other two transgenes based on the sequence similarity.