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验证数据展示

  • WB analysis using 83922-2-RR (same clone as 83922-2-PBS)

    WB analysis using 83922-2-RR (same clone as 83922-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83922-2-RR (transgelin/SM22 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83922-2-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 83922-2-RR (same clone as 83922-2-PBS)

    IHC staining of mouse heart using 83922-2-RR (same clone as 83922-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83922-2-RR (transgelin/SM22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83922-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TAGLN antibody included
  • IHC staining of mouse heart using 83922-2-RR (same clone as 83922-2-PBS)

    IHC staining of mouse heart using 83922-2-RR (same clone as 83922-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83922-2-RR (transgelin/SM22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83922-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TAGLN antibody included
  • IF Staining of mouse heart using 83922-2-RR (same clone as 83922-2-PBS)

    IF Staining of mouse heart using 83922-2-RR (same clone as 83922-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using transgelin/SM22 antibody (83922-2-RR, Clone: 240864A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83922-2-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 83922-2-RR (same clone as 83922-2-PBS)

    IF Staining of mouse heart using 83922-2-RR (same clone as 83922-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using transgelin/SM22 antibody (83922-2-RR, Clone: 240864A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83922-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83922-2-RR

    Affinity and Kinetic Characterization of 83922-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83922-2-RR against Human transgelin/SM22 were performed. The affinity constant is 14.2 nM.

transgelin/SM22 Recombinant antibody, PBS Only

transgelin/SM22 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83922-2-PBS

产品说明书

CloneNo. 240864A11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

SM22, transgelin, SM22-alpha, SM22-a, Protein WS3-10

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy TAGLN Ready-To-Use IHC Kit. TAGLN primary antibody included.

产品信息

83922-2-PBS targets transgelin/SM22 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 transgelin/SM22 fusion protein Ag0764 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 transgelin
别名 SM22, transgelin, SM22-alpha, SM22-a, Protein WS3-10
计算分子量 22 kDa
观测分子量 19-22 kDa
GenBank蛋白编号BC004927
基因名称 transgelin/SM22
Gene ID (NCBI) 6876
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ01995
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The transgelin family is a protein group belonging to the 22kd actin-related calponin superfamily. Of all three isoforms, transgelin 1 is the best characterized. Transgelin 1, or SM22alpha, is a specific marker for differentiated smooth muscle cells. Transgelin 2, also known as SM22 beta, is expressed by both smooth and non-smooth muscle cells in a temporally and spatially regulated pattern. Trangenlin 3, also known as NP25, is only found in highly differentiated neuronal cells. This antibody was generated against full-length transgelin 1 protein. It can cross-react with other two transgenes based on the sequence similarity.