验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HEK-293 cells, COLO 320 cells, HepG2 cells |
| Positive IP detected in | COLO 320 cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy TALDO1 Ready-To-Use IHC Kit. TALDO1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
产品信息
12376-1-AP targets TALDO1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | TALDO1 fusion protein Ag3031 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transaldolase 1 |
| 别名 | transaldolase 1, TALDOR, TALDO, EC:2.2.1.2 |
| 计算分子量 | 337 aa, 38 kDa |
| 观测分子量 | 38 kDa |
| GenBank蛋白编号 | BC009680 |
| 基因名称 | TALDO1 |
| Gene ID (NCBI) | 6888 |
| RRID | AB_2199712 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P37837 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TALDO1 belongs to the transaldolase family which contributes to the generation of NADPH in the nonoxidative phase of the pentose phosphate pathway (PPP). Defects in TALDO1 are the cause of transaldolase 1 deficiency which results in telangiectases of the skin, hepatosplenomegaly, and enlarged clitoris.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TALDO1 antibody 12376-1-AP | Download protocol |
| IHC protocol for TALDO1 antibody 12376-1-AP | Download protocol |
| IP protocol for TALDO1 antibody 12376-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle. | ||
Cancer Cell Nrf2 redirects glucose and glutamine into anabolic pathways in metabolic reprogramming. | ||
Nat Commun Synthetic lethality between HER2 and transaldolase in intrinsically resistant HER2-positive breast cancers.
| ||
J Biol Chem Metabolic reprogramming driven by EZH2 inhibition depends on cell-matrix interactions | ||
Cell Rep A pro-metastatic tRNA fragment drives aldolase A oligomerization to enhance aerobic glycolysis in lung adenocarcinoma |