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验证数据展示

  • WB analysis using 10161-1-AP

    WB analysis using 10161-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10161-1-AP (TAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 10161-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 10161-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10161-1-AP (TAP2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TAP2 antibody included
  • IHC staining of human ovary cancer using 10161-1-AP

    IHC staining of human ovary cancer using 10161-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10161-1-AP (TAP2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TAP2 antibody included

TAP2 Polyclonal antibody

TAP2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10161-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

PSF-2, PSF2, EC:7.4.2.14, ATP-binding cassette sub-family B member 3, APT2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, SH-SY5Y cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TAP2 Ready-To-Use IHC Kit. TAP2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10161-1-AP targets TAP2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 TAP2 fusion protein Ag0190 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transporter 2, ATP-binding cassette, sub-family B (MDR/TAP)
别名 PSF-2, PSF2, EC:7.4.2.14, ATP-binding cassette sub-family B member 3, APT2
计算分子量 76 kDa
观测分子量70 kDa
GenBank蛋白编号BC002751
基因名称 TAP2
Gene ID (NCBI) 6891
RRIDAB_3085365
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ03519
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TAP2, also known as APT2, ABCB3, is a member of the superfamily of ATP-binding cassette (ABC) transporters. TAP2 mediates unidirectional translocation of peptide antigens from cytosol to endoplasmic reticulum (ER) for loading onto MHC class I (MHCI) molecules (PMID: 25656091). Mutations in TAP2 may be associated with Bare lymphocyte syndrome 1 (BLS1) and schizophrenia(PMID: 7517574, 24365204).

实验方案

Product Specific Protocols
WB protocol for TAP2 antibody 10161-1-APDownload protocol
IHC protocol for TAP2 antibody 10161-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols