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TAPT1 Polyclonal antibody

TAPT1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27657-1-AP

Print datasheet

Synonyms

CMVFR, TAPT1

验证数据展示

  • WB analysis using 27657-1-AP

    WB analysis using 27657-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27657-1-AP (TAPT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 27657-1-AP

    IHC staining of human liver cancer using 27657-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27657-1-AP (TAPT1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 27657-1-AP

    IHC staining of human liver cancer using 27657-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27657-1-AP (TAPT1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inNIH/3T3 cells, mouse testis tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

27657-1-AP targets TAPT1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity Human, mouse
Cited Reactivitymouse
Immunogen TAPT1 fusion protein Ag26360 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transmembrane anterior posterior transformation 1
Synonyms CMVFR, TAPT1
Calculated Molecular Weight 567 aa, 64 kDa
Observed Molecular Weight 65~70 kDa
GenBank Accession NumberBC066899
Gene Symbol TAPT1
Gene ID (NCBI) 202018
RRIDAB_2880940
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6NXT6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TAPT1 antibody 27657-1-APDownload protocol
IHC protocol for TAPT1 antibody 27657-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Bone Res

Runx1 protects against the pathological progression of osteoarthritis.

Authors - Chenchen Zhou
View Article