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TARS Polyclonal antibody, PBS Only

TARS Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14773-1-PBS

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Synonyms

验证数据展示

  • WB analysis of A549 using 14773-1-AP (same clone as 14773-1-PBS)

    WB analysis of A549 using 14773-1-AP (same clone as 14773-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 14773-1-AP (TARS antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14773-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 14773-1-AP (same clone as 14773-1-PBS)

    IF Staining of MCF-7 using 14773-1-AP (same clone as 14773-1-PBS)

    Immunofluorescent analysis of MCF-7 cells, using TARS antibody 14773-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14773-1-PBS in a different storage buffer formulation.

  • IP experiment of A549 using 14773-1-AP (same clone as 14773-1-PBS)

    IP experiment of A549 using 14773-1-AP (same clone as 14773-1-PBS)

    IP result of anti-TARS (IP:14773-1-AP, 4ug; Detection:14773-1-AP 1:500) with A549 cells lysate 2400ug. This data was developed using the same antibody clone with 14773-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 14773-1-AP (same clone as 14773-1-PBS)

    IHC staining of human breast cancer using 14773-1-AP (same clone as 14773-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14773-1-AP (TARS Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14773-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 14773-1-AP (same clone as 14773-1-PBS)

    IHC staining of human breast cancer using 14773-1-AP (same clone as 14773-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14773-1-AP (TARS Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14773-1-PBS in a different storage buffer formulation.

产品信息

14773-1-PBS targets TARS in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human
Immunogen TARS fusion protein Ag6614 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name threonyl-tRNA synthetase
Synonyms
Calculated Molecular Weight 83 kDa
Observed Molecular Weight 66-72 kDa
GenBank Accession NumberBC000517
Gene Symbol TARS
Gene ID (NCBI) 6897
RRIDAB_2200654
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP26639
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TARS (Threonyl-tRNA synthetase), also known as threonine-tRNA ligase or ThrRS, belongs to the aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetase (aaRS) catalyzes the specific esterification of an amino acid with the 3'-terminal hydroxyl group of its cognate tRNA. Specificity of aminoacylation catalyzed by aaRSs ensures the accurate translation of the genetic code in the first step of protein synthesis (PMID: 26324710). TARS has some isoforms with the molecular mass of 83, 87 kDa and other MW.