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TARS Monoclonal antibody, PBS Only

TARS Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A11F10

Cat no : 67828-1-PBS

Print datasheet

Synonyms

TARS, Threonine tRNA ligase, threonyl tRNA synthetase, ThrRS

验证数据展示

  • WB analysis using 67828-1-Ig (same clone as 67828-1-PBS)

    WB analysis using 67828-1-Ig (same clone as 67828-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67828-1-Ig (TARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67828-1-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 67828-1-Ig (same clone as 67828-1-PBS)

    WB analysis of HSC-T6 using 67828-1-Ig (same clone as 67828-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67828-1-Ig (TARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67828-1-PBS in a different storage buffer formulation.

  • WB analysis of 4T1 using 67828-1-Ig (same clone as 67828-1-PBS)

    WB analysis of 4T1 using 67828-1-Ig (same clone as 67828-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67828-1-Ig (TARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67828-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67828-1-Ig (same clone as 67828-1-PBS)

    IF Staining of HeLa using 67828-1-Ig (same clone as 67828-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TARS antibody (67828-1-Ig, Clone: 1A11F10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67828-1-PBS in a different storage buffer formulation.

产品信息

67828-1-PBS targets TARS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen TARS fusion protein Ag6614 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name threonyl-tRNA synthetase
Synonyms TARS, Threonine tRNA ligase, threonyl tRNA synthetase, ThrRS
Calculated Molecular Weight 83 kDa
Observed Molecular Weight 83-87 kDa
GenBank Accession NumberBC000517
Gene Symbol TARS
Gene ID (NCBI) 6897
RRIDAB_2918590
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP26639
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TARS (Threonyl-tRNA synthetase), also known as threonine-tRNA ligase or ThrRS, belongs to the aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetase (aaRS) catalyzes the specific esterification of an amino acid with the 3'-terminal hydroxyl group of its cognate tRNA. Specificity of aminoacylation catalyzed by aaRSs ensures the accurate translation of the genetic code in the first step of protein synthesis (PMID: 26324710). TARS has some isoforms with the molecular mass of 83, 87 kDa and other MW.