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验证数据展示

  • WB analysis using 67828-1-Ig (same clone as 67828-1-PBS)

    WB analysis using 67828-1-Ig (same clone as 67828-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67828-1-Ig (TARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67828-1-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 67828-1-Ig (same clone as 67828-1-PBS)

    WB analysis of HSC-T6 using 67828-1-Ig (same clone as 67828-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67828-1-Ig (TARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67828-1-PBS in a different storage buffer formulation.

  • WB analysis of 4T1 using 67828-1-Ig (same clone as 67828-1-PBS)

    WB analysis of 4T1 using 67828-1-Ig (same clone as 67828-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67828-1-Ig (TARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67828-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67828-1-Ig (same clone as 67828-1-PBS)

    IF Staining of HeLa using 67828-1-Ig (same clone as 67828-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TARS antibody (67828-1-Ig, Clone: 1A11F10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67828-1-PBS in a different storage buffer formulation.

TARS Monoclonal antibody, PBS Only

TARS Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67828-1-PBS

产品说明书

CloneNo. 1A11F10

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

TARS, Threonine tRNA ligase, threonyl tRNA synthetase, ThrRS

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67828-1-PBS targets TARS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag6614

Product name: Recombinant human TARS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 394-723 aa of BC000517

Sequence: ENMFSFEVEKELFALKPMNCPGHCLMFDHRPRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAMEQIEDEIKGCLDFLRTVYSVFGFSFKLNLSTRPEKFLGDIEVWDQAEKQLENSLNEFGEKWELNSGDGAFYGPKIDIQIKDAIGRYHQCATIQLDFQLPIRFNLTYVSHDGDDKKRPVIVHRAILGSVERMIAILTENYGGKWPFWLSPRQVMVVPVGPTCDEYAQKVRQQFHDAKFMADIDLDPGCTLNKKIRNAQLAQYNFILVVGEKEKISGTVNIRTRDNKVHGERTISETIERLQQLKEFRSKQAEEEF

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 threonyl-tRNA synthetase
别名 TARS, Threonine tRNA ligase, threonyl tRNA synthetase, ThrRS
计算分子量 83 kDa
观测分子量 83-87 kDa
GenBank蛋白编号BC000517
基因名称 TARS
Gene ID (NCBI) 6897
RRIDAB_2918590
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP26639
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TARS (Threonyl-tRNA synthetase), also known as threonine-tRNA ligase or ThrRS, belongs to the aminoacyl-tRNA synthetase family. Aminoacyl-tRNA synthetase (aaRS) catalyzes the specific esterification of an amino acid with the 3'-terminal hydroxyl group of its cognate tRNA. Specificity of aminoacylation catalyzed by aaRSs ensures the accurate translation of the genetic code in the first step of protein synthesis (PMID: 26324710). TARS has some isoforms with the molecular mass of 83, 87 kDa and other MW.

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