验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells, mouse liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | mouse heart tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15067-1-AP targets TARS2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | TARS2 fusion protein Ag7074 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | threonyl-tRNA synthetase 2, mitochondrial (putative) |
| 别名 | Threonyl-tRNA synthetase-like 1, Threonyl-tRNA synthetase, Threonine--tRNA ligase, mitochondrial, Threonine tRNA ligase, TARSL1 |
| 计算分子量 | 81 kDa |
| 观测分子量 | 81 kDa |
| GenBank蛋白编号 | BC000541 |
| 基因名称 | TARS2 |
| Gene ID (NCBI) | 80222 |
| RRID | AB_2200668 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BW92 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TARS2 antibody 15067-1-AP | Download protocol |
| IHC protocol for TARS2 antibody 15067-1-AP | Download protocol |
| IF protocol for TARS2 antibody 15067-1-AP | Download protocol |
| IP protocol for TARS2 antibody 15067-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell Mitochondrial Threonyl-tRNA Synthetase TARS2 Is Required for Threonine-Sensitive mTORC1 Activation.
| ||
Bioengineered Knockdown of mitochondrial threonyl-tRNA synthetase 2 inhibits lung adenocarcinoma cell proliferation and induces apoptosis.
| ||
Redox Biol Decreased expression of mitochondrial aminoacyl-tRNA synthetases causes downregulation of OXPHOS subunits in type 2 diabetic muscle | ||
Int J Biol Markers Potential prognostic biomarkers of hepatocellular carcinoma based on 4D label-free quantitative proteomics analysis pilot investigation |
