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TARSL2 Polyclonal antibody

TARSL2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24635-1-AP

Print datasheet

Synonyms

Threonyl-tRNA synthetase protein 3, Threonyl-tRNA synthetase, Threonine--tRNA ligase 2, cytoplasmic, Threonine  tRNA ligase, TARS3

验证数据展示

  • WB analysis of L02 using 24635-1-AP

    WB analysis of L02 using 24635-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 24635-1-AP (TARSL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 24635-1-AP

    IHC staining of human brain using 24635-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24635-1-AP (TARSL2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 24635-1-AP

    IHC staining of human brain using 24635-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24635-1-AP (TARSL2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of L02 using 24635-1-AP

    IF Staining of L02 using 24635-1-AP

    Immunofluorescent analysis of L02 cells using 24635-1-AP (TARSL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inL02 cells, HEK-293 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inL02 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24635-1-AP targets TARSL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen TARSL2 fusion protein Ag18298 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name threonyl-tRNA synthetase-like 2
Synonyms Threonyl-tRNA synthetase protein 3, Threonyl-tRNA synthetase, Threonine--tRNA ligase 2, cytoplasmic, Threonine  tRNA ligase, TARS3
Calculated Molecular Weight 802 aa, 93 kDa
Observed Molecular Weight 93 kDa
GenBank Accession NumberBC132671
Gene Symbol TARSL2
Gene ID (NCBI) 123283
RRIDAB_2879648
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA2RTX5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TARSL2 antibody 24635-1-APDownload protocol
IHC protocol for TARSL2 antibody 24635-1-APDownload protocol
IF protocol for TARSL2 antibody 24635-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols