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验证数据展示

  • WB analysis using 17252-1-AP

    WB analysis using 17252-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17252-1-AP (TBC1D15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis using 17252-1-AP

    WB analysis using 17252-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17252-1-AP (TBC1D15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 17252-1-AP

    IF Staining of HeLa using 17252-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TBC1D15 antibody (17252-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

TBC1D15 Polyclonal antibody

TBC1D15 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17252-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA and More (1)

别名

TBC1 domain family member 15, Rab7-GAP, Rab7 GAP, GTPase-activating protein RAB7, GTPase activating protein RAB7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293T cells, A431 cells, mouse liver tissue, rat liver tissue
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TBC1D15 Ready-To-Use IHC Kit. TBC1D15 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

17252-1-AP targets TBC1D15 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 TBC1D15 fusion protein Ag10625 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TBC1 domain family, member 15
别名 TBC1 domain family member 15, Rab7-GAP, Rab7 GAP, GTPase-activating protein RAB7, GTPase activating protein RAB7
计算分子量 691 aa, 79 kDa
观测分子量 79 kDa
GenBank蛋白编号BC028352
基因名称 TBC1D15
Gene ID (NCBI) 64786
RRIDAB_2878370
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TC07
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TBC1D15 is a highly conserved protein belonging to the TBC domain family and has similar functions to Rab-GAP. TBC1D15 is ubiquitously expressed and localized predominantly to the cytosol. TBC1D15 is a protein that contains a conserved Tre2/Bub2/Cdc16 (TBC) domain. TBC1D15 can serve as a mitochondrial Rab GTPase-activating protein (Rab-GAP) and influence autophagosome biogenesis and morphology downstream of Parkin activation. TBC1D15 can also control stem cell self-renewal when coupled with the Numb-p53 complex.

实验方案

Product Specific Protocols
WB protocol for TBC1D15 antibody 17252-1-APDownload protocol
IF protocol for TBC1D15 antibody 17252-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cell Death Dis

SENP3-FIS1 axis promotes mitophagy and cell survival under hypoxia

Authors - Alice Zhao
View Article