验证数据展示
经过测试的应用
Positive WB detected in | U2OS cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, pig brain tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68409-1-Ig targets TBC1D23 in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | Human, mouse, rat, pig |
免疫原 | TBC1D23 fusion protein Ag10727 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | TBC1 domain family, member 23 |
别名 | DKFZp667G062, FLJ11046, NS4ATP1, TBC1 domain family member 23, TBC1 domain family, member 23, TBC1D23 |
计算分子量 | 699 aa, 78 kDa |
观测分子量 | 78 kDa |
GenBank蛋白编号 | BC010221 |
基因名称 | TBC1D23 |
Gene ID (NCBI) | 55773 |
RRID | AB_3085126 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q9NUY8 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TBC1D23, also named as NS4ATP1, is a gene down-regulated by Pneumocystis infection. Pneumocystis pneumonia is a common opportunistic disease in AIDS patients. (PMID: 20377877) Recently it has been found that TBC1D23 gene is the most frequently mutated in MSI-H cell lines and protein expressions of TBC1D23 in tumors with mutation were down regulated.(PMID: 20824714)
实验方案
Product Specific Protocols | |
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WB protocol for TBC1D23 antibody 68409-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |